Human G-CSF DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human G-CSF DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CSF3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human G-CSF DuoSet ELISA, 15 Plate

• C17orf33
• chromosome 17 open reading frame 33
• colony stimulating factor 3 (granulocyte)
• CSF3
• CSF3OS
• Filgrastim
• GCSF
• G-CSF
• GCSFlenograstim
• granulocyte colony-stimulating factor
• Lenograstim
• MGC45931
• Pluripoietin

Background

Granulocyte-colony stimulating factor (G-CSF) is a 24-25 kDa monomeric glycoprotein that regulates the proliferation, differentiation, and activation of hematopoietic cells in the neutrophilic granulocyte lineage. Mature human G-CSF is a 178 amino acid (aa) O-glycosylated protein that contains two intrachain disulfide bridges. In humans, alternate splicing generates a second minor isoform with a 3 aa deletion. Mouse and human G-CSF share 76% aa sequence identity, and the two proteins show species cross-reactivity. G-CSF is produced by activated monocytes and macrophages, fibroblasts, endothelial cells, astrocytes, neurons, and bone marrow stroma cells. In addition, various tumor cells express G-CSF constitutively.   

Human G-CSF receptor (G-CSF R) is a 120 kDa type I transmembrane glycoprotein that belongs to the hematopoietin receptor superfamily. The mature protein consists of a 603 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 186 aa cytoplasmic domain. The ECD contains an N-terminal Ig-like domain, a cytokine receptor homology domain, and three fibronectin type III domains. Alternate splicing of human G-CSF R generates additional isoforms including a potentially soluble form of the receptor. The ECDs of mouse and human G-CSF R share 63% aa sequence identity. G-CSF R forms a complex with the ligand in a 2:2 ratio. It is expressed on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types.   

G-CSF is an important regulator for granulopoiesis in vivo, and mutations in G-CSF R are associated with congenital neutropenia. G-CSF can support the growth of multilineage hematopoietic progenitor cells and mobilize them from the bone marrow into the bloodstream. G-CSF enhances the functional capacity of mature neutrophils and supports their survival by limiting the rate of apoptosis. G-CSF also enhances M-CSF induced monocytopoiesis from hematopoietic progenitor cells and stimulates the proliferation of peripheral Th2-inducing dendritic cells (30, 31). It promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia.

Publications for G-CSF (DY214)(23)

Publications using DY214

• Shepelkova, GS;Evstifeev, VV;Berezovskiy, YS;Ergeshova, AE;Tarasov, RV;Bagirov, MA;Yeremeev, VV; Characteristics of Pulmonary Inflammation in Patients with Different Forms of Active Tuberculosis International journal of molecular sciences 2024-11-02 [PMID: 39519346] (Human)
• Hampton, TH;Barnaby, R;Roche, C;Nymon, A;Fukutani, KF;MacKenzie, TA;Stanton, BA; Gene expression responses of CF airway epithelial cells exposed to elexacaftor/tezacaftor/ivacaftor (ETI) suggest benefits beyond improved CFTR channel function bioRxiv : the preprint server for biology 2024-08-29 [PMID: 39257747] (Human)
• Gysan, MR;Milacek, C;Bal, C;Zech, A;Brugger, J;Milos, RI;Antoniewicz, L;Idzko, M;Gompelmann, D; Ventilatory support and inflammatory peptides in hospitalised patients with COVID-19: A prospective cohort trial PloS one 2023-11-02 [PMID: 37917760] (Human)
• Jiang, J;Altammar, J;Cong, X;Ramsauer, L;Steinbacher, V;Dornseifer, U;Schilling, AF;Machens, HG;Moog, P; Hypoxia Preconditioned Serum (HPS) Promotes Proliferation and Chondrogenic Phenotype of Chondrocytes In Vitro International journal of molecular sciences 2023-06-21 [PMID: 37445617] (Human)
• Liu, Y;Smith, MR;Wang, Y;D'Agostino, R;Ruiz, J;Lycan, T;Kucera, GL;Miller, LD;Li, W;Chan, MD;Farris, M;Su, J;Song, Q;Zhao, D;Chandrasekaran, A;Xing, F; c-Met Mediated Cytokine Network Promotes Brain Metastasis of Breast Cancer by Remodeling Neutrophil Activities Cancers 2023-05-05 [PMID: 37174093]
• SR Bruno, A Kumar, ZF Mark, R Chandrasek, E Nakada, N Chamberlai, B Mihavics, J Walzer, J Cahoon, AE Dixon, B Cunniff, V Anathy DRP1-Mediated Mitochondrial Fission Regulates Lung Epithelial Response to Allergen International Journal of Molecular Sciences, 2021-10-15;22(20):. 2021-10-15 [PMID: 34681784] (Human)
• M Lesouhaiti, M Gregoire, A Gacouin, V Coirier, A Frerou, C Piau, V Cattoir, E Dumontet, M Revest, P Tattevin, A Roisne, JP Verhoye, E Flecher, Y Le Tulzo, K Tarte, JM Tadié Neutrophil function and bactericidal activity against Staphylococcus aureus after cardiac surgery with cardiopulmonary bypass Journal of leukocyte biology, 2021-08-23;0(0):. 2021-08-23 [PMID: 34425029] (Human)
• LE Cabrera, PT Pekkarinen, M Alander, KHA Nowlan, NA Nguyen, S Jokiranta, S Kuivanen, A Patjas, S Mero, SH Pakkanen, S Heinonen, A Kantele, O Vapalahti, E Kekäläinen, T Strandin Characterization of low-density granulocytes in COVID-19 PloS Pathogens, 2021-07-06;17(7):e1009721. 2021-07-06 [PMID: 34228753] (Human)
• PK Fazeli, MA Bredella, OG Pachon-Peñ, W Zhao, X Zhang, AT Faje, M Resulaj, SP Polineni, TM Holmes, H Lee, EK O'Donnell, OA MacDougald, MC Horowitz, CJ Rosen, A Klibanski The dynamics of human bone marrow adipose tissue in response to feeding and fasting JCI Insight, 2021-06-22;0(0):. 2021-06-22 [PMID: 33974568] (Human)
• J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy Nature Communications, 2021-02-17;12(1):1087. 2021-02-17 [PMID: 33597530] (Human)
• AER Oliveira, MCA Pereira, AT Belew, LRP Ferreira, LMN Pereira, EGA Neves, MDCP Nunes, BA Burleigh, WO Dutra, NM El-Sayed, RT Gazzinelli, SMR Teixeira Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi�strains unveil a role for fibroblasts in neutrophil recruitment and activation PLoS Pathog., 2020-08-18;16(8):e1008781. 2020-08-18 [PMID: 32810179] (Human)
• L Mercurio, CM Failla, L Capriotti, C Scarponi, F Facchiano, M Morelli, S Rossi, G Pagnanelli, C Albanesi, A Cavani, S Madonna Interleukin (IL)-17/IL-36 axis participates to the crosstalk between endothelial cells and keratinocytes during inflammatory skin responses PLoS ONE, 2020-04-30;15(4):e0222969. 2020-04-30 [PMID: 32352958] (Human)
• B Kim, O Shynlova, S Lye Probiotic Lactobacillus rhamnosus GR-1 is a unique prophylactic agent that suppresses infection-induced myometrial cell responses Sci Rep, 2019-03-18;9(1):4698. 2019-03-18 [PMID: 30886179] (Human)
• J Saliba, B Coutaud, V Solovieva, F Lu, V Blank Regulation of CXCL1 chemokine and CSF3 cytokine levels in myometrial cells by the MAFF transcription factor J. Cell. Mol. Med., 2019-01-22;0(0):. 2019-01-22 [PMID: 30669188] (Human)
• E Redondo-Ca, C Cunningham, J Miller, L Martuscell, S Aoulad-Ali, NJ Rothwell, CM Kielty, SM Allan, E Pinteaux Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro Stem Cell Res Ther, 2017-04-17;8(1):79. 2017-04-17 [PMID: 28412968] (Human)
• IL-17 Promotes Neutrophil-Mediated Immunity by Activating Microvascular Pericytes and Not Endothelium J Immunol, 2016-08-17;0(0):. 2016-08-17 [PMID: 27534549] (Human)
• David M, Kelly E, Zoellner H Opposite cytokine synthesis by fibroblasts in contact co-culture with osteosarcoma cells compared with transwell co-cultures. Cytokine, 2013-03-19;62(1):48-51. 2013-03-19 [PMID: 23523091] (Human)
• Ramirez-Carrozzi V, Sambandam A, Luis E IL-17C regulates the innate immune function of epithelial cells in an autocrine manner. Nat. Immunol., 2011-10-12;12(12):1159-66. 2011-10-12 [PMID: 21993848] (Human)
• Rebelo SL, Amel-Kashipaz MR, Radford PM, Bainbridge SE, Fiets R, Fang J, McDermott EM, Powell RJ, Todd I, Tighe PJ Novel markers of inflammation identified in tumor necrosis factor receptor-associated periodic syndrome (TRAPS) by transcriptomic analysis of effects of TRAPS-associated tumor necrosis factor receptor type I mutations in an endothelial cell line. Arthritis Rheum., 2009-01-01;60(1):269-80. 2009-01-01 [PMID: 19116900] (Human)
• Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. J. Clin. Invest., 2007-12-01;117(12):3664-3672. 2007-12-01 [PMID: 17992260] (Human)
• Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, Wisniewska-Elnur J, Pawlaczyk K, Trominska J, Breborowicz A, Jorres A Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum. Kidney Int., 2007-01-17;71(6):514-25. 2007-01-17 [PMID: 17228364] (Human)
• Clarke DL, Belvisi MG, Smith SJ, Hardaker E, Yacoub MH, Meja KK, Newton R, Slater DM, Giembycz MA Prostanoid receptor expression by human airway smooth muscle cells and regulation of the secretion of granulocyte colony-stimulating factor. Am. J. Physiol. Lung Cell Mol. Physiol., 2005-02-01;288(2):L238-50. 2005-02-01 [PMID: 15640521] (Human)
• Channon JY, Miselis KA, Minns LA, Dutta C, Kasper LH Toxoplasma gondii induces granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor secretion by human fibroblasts: implications for neutrophil apoptosis. Infect. Immun., 2002-11-01;70(11):6048-57. 2002-11-01 [PMID: 12379681] (Human)

FAQs for G-CSF (DY214). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CSF3