Human Fas/TNFRSF6/CD95 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Fas/TNFRSF6/CD95 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FAS

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Fas/TNFRSF6/CD95 DuoSet ELISA, 15 Plate

• Apo-1 antigen
• Apo-1
• apoptosis antigen 1
• Apoptosis-mediating surface antigen FAS
• APT1
• APT1FASTM
• CD95 antigen
• CD95
• CD95ALPS1A
• Fas (TNF receptor superfamily, member 6)
• Fas AMA
• Fas antigen
• Fas
• FAS1
• FASLG receptor
• TNFRSF6
• TNFRSF6member 6
• tumor necrosis factor receptor superfamily member 6

Background

Fas (Fibroblast-associated), also known as Apo-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein that binds to monoclonal antibodies that are cytolytic for various human cell lines. Alternatively spliced cDNAs encoding multiple Fas isoforms, including a soluble form of Fas, have been identified. Fas is highly expressed in epithelial cells, hepatocyes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary.

Publications for Fas/TNFRSF6/CD95 (DY326)(6)

Publications using DY326

• Lindrova, I;Kolackova, M;Svadlakova, T;Vankova, R;Chmelarova, M;Rosecka, M;Jozifkova, E;Sembera, M;Krejsek, J;Slezak, R; Unsolved mystery of Fas: mononuclear cells may have trouble dying in patients with Sjögren's syndrome BMC immunology 2023-06-23 [PMID: 37353767] (Human)
• P Bajgain, AG Torres Cha, K Balasubram, L Fleckenste, P Lulla, HE Heslop, J Vera, AM Leen Secreted Fas decoys enhance the antitumor activity of engineered and bystander T cells in Fas ligand-expressing solid tumors Oncogene, 2022-11-02;0(0):. 2022-11-02 [PMID: 36122411] (Human)
• C Ehrnthalle, S Braumüller, S Kellermann, F Gebhard, M Perl, M Huber-Lang Complement Factor C5a Inhibits Apoptosis of Neutrophils-A Mechanism in Polytrauma? Journal of Clinical Medicine, 2021-07-17;10(14):. 2021-07-17 [PMID: 34300323] (Human)
• HJ Kim, KH Park, YM Kim, E Joo, K Ahn, S Shin A protein microarray analysis of amniotic fluid proteins for the prediction of spontaneous preterm delivery in women with preterm premature rupture of membranes at 23 to 30 weeks of gestation PLoS ONE, 2020-12-31;15(12):e0244720. 2020-12-31 [PMID: 33382822] (Human)
• Yamanegi K, Yamane J, Kobayashi K Valproic acid cooperates with hydralazine to augment the susceptibility of human osteosarcoma cells to Fas- and NK cell-mediated cell death. Int. J. Oncol., 2012-04-19;41(1):83-91. 2012-04-19 [PMID: 22576685] (Human)
• Hsu HY, Wittemann S, Schneider EM, Weiss M, Joos TO Suspension microarrays for the identification of the response patterns in hyperinflammatory diseases. Med Eng Phys, 2008-03-03;30(8):976-83. 2008-03-03 [PMID: 18313970] (Human)

FAQs for Fas/TNFRSF6/CD95 (DY326). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FAS