Human CXCL2/GRO beta DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human CXCL2/GRO beta DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CXCL2/GRO beta DuoSet ELISA, 5 Plate

• chemokine (C-X-C motif) ligand 2
• CINC-2a
• CINC3
• CINC-3
• C-X-C Motif Chemokine 2
• CXCL2
• GRO beta
• GRO2 oncogene
• GRO2
• GROB
• Gro-beta
• Growth-regulated protein beta
• Macrophage inflammatory protein 2-alpha
• melanoma growth stimulatory activity beta
• MGSA beta
• MGSA-b
• MGSA-beta
• MIP2
• MIP-2
• MIP2A
• MIP-2a
• MIP2-alpha
• SCYB2

Background

CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.

Publications for CXCL2/GRO beta/MIP-2/CINC-3 (DY276-05)(14)

Publications using DY276-05

• Shepelkova, GS;Evstifeev, VV;Berezovskiy, YS;Ergeshova, AE;Tarasov, RV;Bagirov, MA;Yeremeev, VV; Characteristics of Pulmonary Inflammation in Patients with Different Forms of Active Tuberculosis International journal of molecular sciences 2024-11-02 [PMID: 39519346] (Human)
• Virard, F;Giraud, S;Bonnet, M;Magadoux, L;Martin, L;Pham, TH;Skafi, N;Deneuve, S;Frem, R;Villoutreix, BO;Sleiman, NH;Reboulet, J;Merabet, S;Chaptal, V;Chaveroux, C;Hussein, N;Aznar, N;Fenouil, T;Treilleux, I;Saintigny, P;Ansieau, S;Manié, S;Lebecque, S;Renno, T;Coste, I; Targeting ERK-MYD88 interaction leads to ERK dysregulation and immunogenic cancer cell death Nature communications 2024-08-15 [PMID: 39147750] (Human)
• Zobel, CM;Wenzel, W;Krüger, JP;Baumgarten, U;Wagelöhner, T;Neumann, N;Foroutan, B;Müller, R;Müller, A;Rauschning, D;Schü beta ler, M;Scheit, L;Weinreich, F;Oltmanns, K;Keidel, F;Koch, M;Spethmann, S;Schreiner, M; Serum interleukin-6, procalcitonin, and C-reactive protein at hospital admission can identify patients at low risk for severe COVID-19 progression Frontiers in microbiology 2023-10-23 [PMID: 37937220] (Human)
• Liu, Y;Smith, MR;Wang, Y;D'Agostino, R;Ruiz, J;Lycan, T;Kucera, GL;Miller, LD;Li, W;Chan, MD;Farris, M;Su, J;Song, Q;Zhao, D;Chandrasekaran, A;Xing, F; c-Met Mediated Cytokine Network Promotes Brain Metastasis of Breast Cancer by Remodeling Neutrophil Activities Cancers 2023-05-05 [PMID: 37174093]
• J Yang, L Jin, HS Kim, F Tian, Z Yi, K Bedi, M Ljungman, M Pasca di M, H Crawford, J Shi KDM6A Loss Recruits Tumor-Associated Neutrophils and Promotes Neutrophil Extracellular Trap Formation in Pancreatic Cancer Cancer Research, 2022-11-15;0(0):OF1-OF14. 2022-11-15 [PMID: 36306422] (Human)
• MS Shaban, C Müller, C Mayr-Buro, H Weiser, J Meier-Soel, BV Albert, A Weber, U Linne, T Hain, I Babayev, N Karl, N Hofmann, S Becker, S Herold, ML Schmitz, J Ziebuhr, M Kracht Multi-level inhibition of coronavirus replication by chemical ER stress Nature Communications, 2021-09-20;12(1):5536. 2021-09-20 [PMID: 34545074] (Human)
• HK Skovdahl, S Gopalakris, TD Svendsen, AVB Granlund, I Bakke, ZG Ginbot, S Thorsvik, A Flatberg, B Sporsheim, J Ostrop, TE Mollnes, AK Sandvik, T Bruland Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration Frontiers in Pharmacology, 2021-05-13;12(0):679741. 2021-05-13 [PMID: 34054553] (Human)
• ID Green, N Pinello, R Song, Q Lee, JM Halstead, CT Kwok, ACH Wong, SS Nair, SJ Clark, B Roediger, U Schmitz, M Larance, R Hayashi, JEJ Rasko, JJ Wong Macrophage development and activation involve coordinated intron retention in key inflammatory regulators Nucleic Acids Res., 2020-07-09;0(0):. 2020-07-09 [PMID: 32449925] (Human)
• L Kloss, C Dollt, K Schledzews, A Krewer, S Melchers, C Manta, C Sticht, C Torre, J Utikal, V Umansky, A Schmieder ADP secreted by dying melanoma cells mediates chemotaxis and chemokine secretion of macrophages via the purinergic receptor P2Y12 Cell Death Dis, 2019-10-07;10(10):760. 2019-10-07 [PMID: 31591378] (Human)
• S Giuliano, M Dufies, PD Ndiaye, J Viotti, D Borchielli, J Parola, V Vial, Y Cormerais, M Ohanna, V Imbert, E Chamorey, N Rioux-Lecl, A Savina, JM Ferrero, B Mograbi, G Pagès Resistance to lysosomotropic drugs used to treat kidney and breast cancers involves autophagy and inflammation and converges in inducing CXCL5 Theranostics, 2019-01-30;9(4):1181-1199. 2019-01-30 [PMID: 30867824] (Human)
• SA Ingelse, J Juschten, MAW Maas, G Matute-Bel, NP Juffermans, JBM van Woense, RA Bem Fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats PLoS ONE, 2019-01-17;14(1):e0210172. 2019-01-17 [PMID: 30653512] (Human)
• A Zukauskas, RJ Mrsny, P Cortés Bar, JR Turner, JM Leong, BA McCormick Transporters MRP1 and MRP2 Regulate Opposing Inflammatory Signals To Control Transepithelial Neutrophil Migration during Streptococcus pneumoniae Lung Infection mSphere, 2018-07-05;3(4):. 2018-07-05 [PMID: 29976647] (Human)
• F Olaru, T Döbel, AS Lonsdorf, S Oehrl, M Maas, AH Enk, M Schmitz, EF Gröne, HJ Gröne, K Schäkel Intracapillary immune complexes recruit and activate slan-expressing CD16+ monocytes in human lupus nephritis JCI Insight, 2018-06-07;3(11):. 2018-06-07 [PMID: 29875315] (Human)
• Xian K Zhang The Fli-1 transcription factor is a critical regulator for controlling the expression of chemokine C-X-C motif ligand 2 (CXCL2) Mol. Immunol., 2016-11-24;81(0):59-66. 2016-11-24 [PMID: 27889620] (Human)

FAQs for CXCL2/GRO beta/MIP-2/CINC-3 (DY276-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.