Human CTGF/CCN2 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human CTGF/CCN2 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CTGF/CCN2 DuoSet ELISA, 5 Plate

• CCN2
• CCN2IGFBP-8
• connective tissue growth factor
• CTGF
• CTGRP
• Fisp12
• HCS24
• Hypertrophic chondrocyte-specific protein 24
• IBP-8
• IGF-binding protein 8
• IGFBP-8
• IGFBP8CCN family member 2
• Insulin-like growth factor-binding protein 8
• MGC102839
• NOV2

Background

CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a common multimodular organization. Each protein contains an IGF-binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF has the ability to bind multiple ligands and has numerous effects on development, differentation, and disease. The C-terminal cysteine knot motif contains the heparin and low density lipoprotein receptor (LDLR) binding sites that contribute to the adhesive and mitogenic properties of CTGF.

Publications for CTGF/CCN2 (DY9190-05)(14)

Publications using DY9190-05

• Yao, X;Chen, Q;Wang, X;Liu, X;Zhang, L; IL-25 induces airway remodeling in asthma by orchestrating the phenotypic changes of epithelial cell and fibrocyte Respiratory research 2023-08-27 [PMID: 37635231] (Human)
• SR Trampuž, S van Riet, Å Nordling, M Ingelman-S The Role of CTGF in Liver Fibrosis Induced in 3D Human Liver Spheroids Cells, 2023-01-13;12(2):. 2023-01-13 [PMID: 36672237] (Human)
• KEC Blokland, M Nizamoglu, H Habibie, T Borghuis, M Schuliga, BN Melgert, DA Knight, CA Brandsma, SD Pouwels, JK Burgess Substrate stiffness engineered to replicate disease conditions influence senescence and fibrotic responses in primary lung fibroblasts Frontiers in Pharmacology, 2022-11-03;13(0):989169. 2022-11-03 [PMID: 36408252] (Human)
• P Wang, Z Huang, Y Peng, H Li, T Lin, Y Zhao, Z Hu, Z Zhou, W Zhou, Y Liu, FF Hou Circular RNA circBNC2 inhibits epithelial cell G2-M arrest to prevent fibrotic maladaptive repair Nature Communications, 2022-10-31;13(1):6502. 2022-10-31 [PMID: 36316334] (Human)
• B Jaeger, JC Schupp, L Plappert, O Terwolbeck, N Artysh, G Kayser, P Engelhard, TS Adams, R Zweigerdt, H Kempf, S Lienenklau, W Garrels, I Nazarenko, D Jonigk, M Wygrecka, D Klatt, A Schambach, N Kaminski, A Prasse Airway basal cells show a dedifferentiated KRT17highPhenotype and promote fibrosis in idiopathic pulmonary fibrosis Nature Communications, 2022-09-26;13(1):5637. 2022-09-26 [PMID: 36163190] (Human)
• S Levy, AB Kilgallen, CM Korse, MIFJ Oerlemans, JPG Sluijter, LW van Laake, GD Valk, MET Tesselaar Elevated Serotonin and NT-proBNP Levels Predict and Detect Carcinoid Heart Disease in a Large Validation Study Cancers, 2022-05-10;14(10):. 2022-05-10 [PMID: 35625964] (Human)
• Fariba Poosti, Mohammad Ayodhia Soebadi, Helena Crijns, Alexandra De Zutter, Mieke Metzemaekers, Nele Berghmans et al. Inhibition of renal fibrosis with a human CXCL9‐derived glycosaminoglycan‐binding peptide Clinical & Translational Immunology 2022-02-02 [PMID: 35140938] (Human)
• A Kuzumi, A Yoshizaki, KM Matsuda, H Kotani, Y Norimatsu, M Fukayama, S Ebata, T Fukasawa, A Yoshizaki-, Y Asano, K Morikawa, Y Kazoe, K Mawatari, T Kitamori, S Sato Interleukin-31 promotes fibrosis and T helper 2 polarization in systemic sclerosis Nature Communications, 2021-10-12;12(1):5947. 2021-10-12 [PMID: 34642338] (Human)
• KEC Blokland, H Habibie, T Borghuis, GJ Teitsma, M Schuliga, BN Melgert, DA Knight, CA Brandsma, SD Pouwels, JK Burgess Regulation of Cellular Senescence Is Independent from Profibrotic Fibroblast-Deposited ECM Cells, 2021-06-29;10(7):. 2021-06-29 [PMID: 34209854] (Human)
• R Samain, A Brunel, T Douché, M Fanjul, S Cassant-So, J Rochotte, J Cros, C Neuzillet, J Raffenne, C Duluc, A Perraud, J Nigri, V Gigoux, I Bieche, M Ponzo, G Carpentier, I Cascone, R Tomasini, HA Schmid, M Mathonnet, R Nicolle, MP Bousquet, Y Martineau, S Pyronnet, C Jean, C Bousquet Pharmacological normalization of pancreatic cancer-associated fibroblast secretome impairs pro-metastatic cross-talk with macrophages: Stromal CSF-1 facilitates metastasis Cellular and Molecular Gastroenterology and Hepatology, 2021-01-20;0(0):. 2021-01-20 [PMID: 33482394] (Human)
• LM Moreira, A Takawale, M Hulsurkar, DA Menassa, A Antanavici, SK Lahiri, N Mehta, N Evans, C Psarros, P Robinson, AJ Sparrow, MA Gillis, N Ashley, P Naud, J Barallobre, K Theofilato, A Lee, M Norris, MV Clarke, PK Russell, B Casadei, S Bhattachar, JD Zajac, RA Davey, M Sirois, A Mead, A Simmons, M Mayr, R Sayeed, G Krasopoulo, C Redwood, KM Channon, JC Tardif, XHT Wehrens, S Nattel, S Reilly Paracrine signalling by cardiac calcitonin controls atrial fibrogenesis and arrhythmia Nature, 2020-11-04;0(0):. 2020-11-04 [PMID: 33149301] (Human)
• B Sun, M Lian, Y Han, X Mo, W Jiang, Z Qiao, K Dai A 3D-Bioprinted dual growth factor-releasing intervertebral disc scaffold induces nucleus pulposus and annulus fibrosus reconstruction Bioact Mater, 2020-08-14;6(1):179-190. 2020-08-14 [PMID: 32913927] (Rat)
• L Tennyson, M Rytel, S Palcsey, L Meyn, R Liang, P Moalli Characterization of the T Cell Response to Polypropylene Mesh in Women with Complications Am. J. Obstet. Gynecol., 2018-11-09;0(0):. 2018-11-09 [PMID: 30419195] (Human)
• C Lambers, C Kornauth, F Oberndorfe, PM Boehm, M Tamm, W Klepetko, M Roth Mechanism of anti-remodelling action of treprostinil in human pulmonary arterial smooth muscle cells PLoS ONE, 2018-11-01;13(11):e0205195. 2018-11-01 [PMID: 30383775] (Human)

FAQs for CTGF/CCN2 (DY9190-05). (Showing 1 - 2 of 2 FAQs).

1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
   • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.