Human Complement Factor H DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Complement Factor H DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CFH

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Complement Factor H DuoSet ELISA, 15 Plate

• adrenomedullin binding protein
• age-related maculopathy susceptibility 1
• AHUS1
• AMBP1
• ARMD4
• ARMS1
• beta-1H
• beta-1-H-globulin
• beta-1-H-globulin
• CFH
• CFHL3
• Complement Factor H
• factor H
• factor H-like 1
• FH
• FHL1
• H factor 1 (complement)
• H factor 1
• H factor 2 (complement)
• HF
• HF1
• HF1ARMS1
• HF2
• HUS
• HUSMGC88246

Background

Complement Factor H is a 155 kDa glycoprotein that provides critical negative regulation to the alternative pathway of complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets, and circulates in the serum at high concentration. Complement Factor H is composed of 20 SCRs (short consensus repeats), each of which consists of approximately 60 amino acids with four invariant Cys residues. Alternate splicing generates a Complement Factor H isoform that is truncated following SCR7.

Complement Factor H interacts with cell surface polyanions including heparin and sialoglycoproteins, and immobilized Complement Factor H supports the CD11b/CD18 integrin-dependent adhesion of neutrophils. It prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5 convertases, and functions as a cofactor for the C3b inactivator, Factor I.

Short consensus repeats 15-20 (SCR15-20) encompass the primary binding sites for heparin and C3b, as well as for the peptide hormone adrenomedullin. Within SCR15-20, human Complement Factor H shares 60% and 63% amino acid sequence identity with mouse and rat Complement Factor H, respectively. Dozens of mutations clustered in SCR15-20 are associated with atypical hemolytic uremic syndrome, a disorder characterized by anemia, thrombocytopenia, and renal failure. Binding of Complement Factor H to tumor cell-associated dentin matrix protein 1, bone sialoprotein, or osteopontin results in the protection of that cell from complement-mediated lysis. A variety of pathogenic microbes also express Complement Factor H binding molecules that interfere with immune clearance of the infection.

Publications for Complement Factor H (DY4779)(3)

Publications using DY4779

• L Chen, ND Perera, AJ Karoukis, KL Feathers, RR Ali, DA Thompson, AT Fahim Oxidative stress differentially impacts apical and basolateral secretion of angiogenic factors from human iPSC-derived retinal pigment epithelium cells Scientific Reports, 2022-07-26;12(1):12694. 2022-07-26 [PMID: 35882889] (Human)
• K Lashkari, GC Teague, U Beattie, J Betts, S Kumar, MM McLaughlin, FJ López Plasma biomarkers of the amyloid pathway are associated with geographic atrophy secondary to age-related macular degeneration PLoS ONE, 2020-08-07;15(8):e0236283. 2020-08-07 [PMID: 32764794] (Human)
• N Shahini, AE Michelsen, PH Nilsson, K Ekholt, L Gullestad, K Broch, CP Dahl, P Aukrust, T Ueland, TE Mollnes, A Yndestad, MC Louwe The alternative complement pathway is dysregulated in patients with chronic heart failure Sci Rep, 2017-02-14;7(0):42532. 2017-02-14 [PMID: 28195242] (Human)

FAQs for Complement Factor H (DY4779). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CFH