Human CCL18/PARC Quantikine ELISA Kit, New

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CCL18/PARC Quantikine ELISA Kit, New Summary

• Background: The Quantikine Human CCL18/PARC Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human CCL18 in cell culture supernates, tissue lysates, serum, plasma, and urine. It contains E. coli-expressed recombinant human CCL18 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CCL18 showed linear curves that were parallel t...o the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CCL18.
  Show More
• Specificity: Natural and recombinant human CCL18.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL18

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL18/PARC Quantikine ELISA Kit, New

• Alternative macrophage activation-associated CC chemokine 1
• AMAC-1
• AMAC1CC chemokine ligand 18
• AMAC-1Small-inducible cytokine A18
• CCL18
• chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
• CKb7
• DC-CK1
• DC-CK1CC chemokine PARC
• DCCK1Macrophage inflammatory protein 4
• Dendritic cell chemokine 1
• MIP4
• MIP-4
• MIP-4C-C motif chemokine 18
• PARC
• PARCPulmonary and activation-regulated chemokine
• SCYA18chemokine (C-C), dendritic
• small inducible cytokine A18
• small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

Background

Pulmonary and activation-regulated chemokine (PARC), also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid sequence identity). In vitro, PARC mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-gamma.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL18/PARC (DCL180B)(13)

Publications using DCL180B

• Zhu, L;Wu, S;Zong, C;Chen, Y;Sun, C;Zhang, Y;Zhang, X;Shu, X;Lu, X;Wang, G;Peng, Q; A serum biomarker-based index for predicting progressive pulmonary fibrosis in patients with anti-synthetase syndrome: the LOCCM index Respiratory research 2025-11-25 [PMID: 41291658] (Human)
• Wu, N;Xue, C;Yu, S;Wang, Y;Sun, D;Ye, Q; CC-chemokine ligand 18, CXC motif chemokine 13 and osteopontin as biomarkers of silicosis and asbestosis: a prospective observational study Scientific reports 2025-02-25 [PMID: 40000810] (Human)
• MSY Lam, JA Reales-Cal, JR Ow, JJY Aw, D Tan, R Vijayakuma, E Ceccarello, T Tabaglio, YT Lim, WL Chien, F Lai, AT Tanoto, Q Chen, RM Sobota, G Adriani, A Bertoletti, E Guccione, A Pavesi G9a/GLP inhibition during ex vivo lymphocyte expansion increases in vivo cytotoxicity of engineered T cells against hepatocellular carcinoma Nature Communications, 2023-02-02;14(1):563. 2023-02-02 [PMID: 36732506] (Human)
• J Wang, N Wang, Z Zheng, Y Che, M Suzuki, S Kano, J Lu, P Wang, Y Sun, A Homma Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinoma BMC Cancer, 2022-11-24;22(1):1208. 2022-11-24 [PMID: 36424539] (Human)
• W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype Oncogene, 2022-11-22;0(0):. 2022-11-22 [PMID: 36418470] (Human)
• Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model Cell Reports, 2022-04-12;39(2):110670. 2022-04-12 [PMID: 35417708] (Human)
• KJ Gregory, SM Morin, A Kubosiak, J Ser-Dolans, B Schalet, DJ Jerry, SS Schneider The use of patient-derived breast tissue explants to study macrophage polarization and the effects of environmental chemical exposure Immunol. Cell Biol., 2020-09-09;0(0):. 2020-09-09 [PMID: 32713010] (Human)
• W Camu, M Mickunas, JL Veyrune, C Payan, C Garlanda, M Locati, R Juntas-Mor, N Pageot, A Malaspina, U Andreasson, J Kirby, C Suehs, S Saker, C Masseguin, J De Vos, H Zetterberg, PJ Shaw, A Al-Chalabi, PN Leigh, T Tree, G Bensimon Repeated 5-day cycles of low dose aldesleukin in amyotrophic lateral sclerosis (IMODALS): A phase 2a randomised, double-blind, placebo-controlled trial EBioMedicine, 2020-07-07;0(0):102844. 2020-07-07 [PMID: 32651161] (Human)
• J Avouac, A Cauvet, A Steelandt, Y Shirai, M Elhai, M Kuwana, O Distler, Y Allanore Improving risk-stratification of rheumatoid arthritis patients for interstitial lung disease PLoS ONE, 2020-05-08;15(5):e0232978. 2020-05-08 [PMID: 32384128] (Human)
• A Nikonova, M Khaitov, DJ Jackson, S Traub, MB Trujillo-T, DA Kudlay, AS Dvornikov, A Del-Rosari, R Valenta, LA Stanciu, R Khaitov, SL Johnston M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations EBioMedicine, 2020-04-09;54(0):102734. 2020-04-09 [PMID: 32279057] (Human)
• EA Ayaub, K Tandon, M Padwal, J Imani, H Patel, A Dubey, O Mekhael, C Upagupta, A Ayoub, A Dvorkin-Gh, J Murphy, PS Kolb, S Lhotak, JG Dickhout, RC Austin, MR Kolb, CD Richards, K Ask IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages Immunol. Cell Biol., 2018-11-14;0(0):. 2018-11-14 [PMID: 30298952] (Human)
• Lane D, Matte I, Garde-Granger P, Laplante C, Carignan A, Rancourt C, Piche A Inflammation-regulating factors in ascites as predictive biomarkers of drug resistance and progression-free survival in serous epithelial ovarian cancers. BMC Cancer, 2015-07-01;15(0):492. 2015-07-01 [PMID: 26122176] (Human)
• Ngan D, Wilcox P, Aldaabil M, Li Y, Leipsic J, Sin D, Man S The relationship of systemic inflammation to prior hospitalization in adult patients with cystic fibrosis. BMC Pulm Med, 2012-02-14;12(0):3. 2012-02-14 [PMID: 22333132] (Human)

FAQs for CCL18/PARC (DCL180B). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL18