HspA1L Antibody (7D1)

Western Blot: HspA1L Antibody (7D1) [NBP2-75530] - Analysis of HSPA1L on A549 (1) and mouse testis (2) lysate using anti-HSPA1L antibody at 1/10,000 dilution.

Immunohistochemistry-Paraffin: HspA1L Antibody (7D1) [NBP2-75530] - Analysis of paraffin-embedded rat testis tissue using anti-HSPA1L antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HspA1L Antibody (7D1) [NBP2-75530] - Analysis of paraffin-embedded mouse testis tissue using anti-HSPA1L antibody. Counter stained with hematoxylin.

Product Discontinued

HspA1L Antibody (7D1) Summary

• Immunogen: Recombinant protein with Human HspA1L aa 335-639 / 641. (SwissProt: P34931 Human; SwissProt: P16627 Mouse; SwissProt: P55063 Rat)
• Localization: Cytosol. Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA1L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:10000-1:50000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HspA1L Antibody (7D1)

• heat shock 10kDa protein 1-like
• Heat shock 70 kDa protein 1-Hom
• Heat shock 70 kDa protein 1L
• heat shock 70 kDa protein 1-like
• heat shock 70kD protein-like 1
• heat shock 70kDa protein 1-like
• HSP70-1L
• HSP70-HOM
• HSP70T
• hum70t

Background

HSPA1L encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA1L