HSP90 Antibody (3B6)

Images

 
Western Blot: HSP90 Antibody (3B6) [NB120-5455] - Analysis of 50ug of various whole cell lysates and 15ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more
Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for ...read more
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more
Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Staining of human colon cancer tissue sections.
Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
3B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HSP90 Antibody (3B6) Summary

Immunogen
Purified mouse HSP90.
Localization
Cytoplasmic
Specificity
Detects heat shock protein 90 kDa (Hsp 90) from human, mouse and rat tissues. This does not detect chicken Hsp 90.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HSP90AA1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:20
Application Notes
WB: Detects an approx. 90 kDa protein representing Hsp 90 from MCF7 cell extract. ab5455 does not immunoprecipitate Hsp 90 in any form (free or complexed).
Reviewed Applications
Read 1 Review rated 5
using
NB120-5455 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for HSP90 Antibody (3B6)

  • FLJ31884
  • Heat shock 86 kDa
  • heat shock 90kD protein 1, alpha
  • heat shock 90kD protein 1, alpha-like 4
  • heat shock 90kD protein, alpha-like 4
  • heat shock 90kDa protein 1, alpha
  • heat shock protein 90kDa alpha (cytosolic), class A member 1
  • heat shock protein HSP 90-alpha
  • Hsp89
  • HSP90
  • HSP90AHSP86
  • HSP90N
  • HSPC1HSP 86
  • HSPCAHSP89A
  • HSPCAL1
  • HSPCAL4
  • HSPN
  • LAP2
  • Renal carcinoma antigen NY-REN-38

Background

Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP90 Antibody (NB120-5455) (0)

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Review for HSP90 Antibody (NB120-5455) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB120-5455:
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WB
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Images Ratings Applications Species Date Details
Western Blot HSP90 NB120-5455
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reviewed by:
Anonymous
WB Mouse 06/03/2015
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Summary

ApplicationWestern Blot
Sample TestedNIH/3T3 whole cell lysate
SpeciesMouse

Blocking

Blocking DetailsBlocked in 5% BSA in TBS-T (0.1% Tween 20), 1 hour, 25C (room temperature)

Primary Anitbody

Dilution RatioDiluted 1:1000 in 5% BSA in TBS-T (0.1% Tween 20), Incubated overnight (~16h) at 4C.

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-conjugated
Secondary Manufacturer Cat#CST 7074S
Secondary Concentration1:2000, 5% non-fat milk in TBS-T (0.1% Tween 20)

Details

Detection NotesECL detection (Amersham ECL Western Blotting Detection Reagent) and Amersham Hyperfilm ECL; 1-minute exposure

Comments

CommentsStrong signal. No issues with background signal.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP90 Antibody (NB120-5455) (0)

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Secondary Antibodies

 

Isotype Controls

Additional HSP90 Products

HSP90 NB120-5455

Bioinformatics Tool for HSP90 Antibody (NB120-5455)

Discover related pathways, diseases and genes to HSP90 Antibody (NB120-5455). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for HSP90 Antibody (NB120-5455)

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Pathways for HSP90 Antibody (NB120-5455)

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Research Areas for HSP90 Antibody (NB120-5455)

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Blogs on HSP90.

HSP90 - an essential eukaryotic protein with implications for drug development
The heat-shock protein 90 (HSP90) family is a group of highly conserved molecular chaperones with important functions in protein folding and in signal transduction. The HSP90 protein structure is so well conserved that some HSP90 antibodies are rea...  Read full blog post.

Integrin beta 1 binding protein 2
ITGB1BP2 is a muscle-specific protein cloned by a rat created by Branccio's group in Italy that was found to interact with the cytoplasmic domain of integrin beta 11. It is expressed only in heart and skeletal muscle but is not essential for normal de...  Read full blog post.

HSP Antibodies: Novel Therapies for MMP-induced Metastatic Breast Cancer
The matrix metalloproteinases are zinc-dependent protease enzymes which interact with a range of ECM (extracellular matrix) proteins, and are activated by proteolytic cleavage. We at Novus Biologicals offer a wide range of top quality MMP reagents, in...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

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Recent Reviews

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5
1
4
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0
2
0
1
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Anonymous
06/03/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol HSP90AA1
Entrez