HSF2 Antibody (SR2042) - BSA Free

Western Blot: HSF2 Antibody (SR2042) [NBP3-22062] - Western blot analysis using NBP3-22062 in HeLa cell lysate.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: SR2042
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HSF2 Antibody (SR2042) - BSA Free Summary

• Description: Novus Biologicals Rabbit HSF2 Antibody (SR2042) - BSA Free (NBP3-22062) is a recombinant monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human HSF2 (Uniprot #: Q03933)
• Specificity: Detects endogenous levels of total HSF2
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HSF2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HSF2 Antibody (SR2042) - BSA Free

• heat shock factor 2
• heat shock factor protein 2
• heat shock transcription factor 2MGC75048
• HSF 2
• HSF2
• HSTF 2
• HSTF2
• MGC117376
• MGC156196

Background

Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up-regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. HSF2 exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form. Various experiments have suggested that HSF2 may play roles in differentiation and development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSF2