HSD17B4 Overexpression Lysate

Western Blot: HSD17B4 Overexpression Lysate (Adult Normal) [NBL1-11728] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HSD17B4.

• Reactivity: Hu
• Applications: WB

HSD17B4 Overexpression Lysate Summary

• Description:

  HSD17B4 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC200460
  
  Accession#: NM_000414
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: HSD17B4

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 79.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for HSD17B4 Overexpression Lysate

• 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase
• 7-alpha
• D-bifunctional protein
• EDH17B4
• hydroxysteroid (17-beta) dehydrogenase 4
• MPF-2
• peroxisomal multifunctional enzyme type 2,17-beta-HSD IV
• peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV
• peroxisomal
• short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Background

The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for HSD17B4 Lysate (NBL1-11728)(1)

Publication using NBL1-11728

• Hill JL, McIver KB, Katzer K, Foster MT Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue Methods and protocols 2022-04-15 [PMID: 35448699] (WB)

Bioinformatics

• Gene Symbol: HSD17B4