![Western Blot: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - Western blot was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1alpha,beta and gamma diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (kDa) is shown on the left; the expected location of HP1alpha, HP1beta and HP1gamma is indicated on the right.](http://images.novusbio.com/fullsize/HP1-Alpha--Beta--and-Gamma-Antibody-Western-Blot-NBP2-59154-img0001.jpg "Western Blot: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - Western blot was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1alpha,beta and gamma diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (kDa) is shown on the left; the expected location of HP1alpha, HP1beta and HP1gamma is indicated on the right.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | The exact immunogen is propietary information. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CBX5 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide and 0.05% ProClin 300 |
| Purity | Protein G purified |
![Immunohistochemistry-Paraffin KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0013.jpg)
![Immunohistochemistry-Paraffin KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0014.jpg)
![Immunocytochemistry/ Immunofluorescence KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2350-img0015.jpg)
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CBX5 |
![Chromatin Immunoprecipitation: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - ChIP assays were performed using NIH3T3 cells and 4 ug of the antibody directed against HP1alpha,beta and gamma. QPCR was performed on the IP'd DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Figure shows the relative enrichment as compared to a no antibody negative control ChIP.](http://images.novusbio.com/fullsize/HP1-Alpha--Beta--and-Gamma-Antibody-Chromatin-Immunoprecipitation-NBP2-59154-img0002.jpg "Chromatin Immunoprecipitation: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - ChIP assays were performed using NIH3T3 cells and 4 ug of the antibody directed against HP1alpha,beta and gamma. QPCR was performed on the IP'd DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Figure shows the relative enrichment as compared to a no antibody negative control ChIP.")
![Immunofluorescence: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - HeLa cells were stained with the antibody against HP1alpha, beta and gamma; and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1alpha,beta and gamma antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/HP1-Alpha-Beta-and-Gamma-Antibody-Immunofluorescence-NBP2-59154-img0003.jpg "Immunofluorescence: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - HeLa cells were stained with the antibody against HP1alpha, beta and gamma; and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1alpha,beta and gamma antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![N/A Haptoglobin [HRP]](https://images.novusbio.com/images2/Haptoglobin_DHAPG0_ELISA_461.jpg)
![N/A Haptoglobin [HRP]](https://images.novusbio.com/images2/DATA_Haptoglobin_DHAPG0_ELISA_709.jpg)
![Western Blot G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241252118.jpg)
![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-412024125214.jpg)
![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241251265.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")