HP1 alpha Antibody

Images

 
Western Blot: HP1 alpha Antibody [NB110-40623] - Whole cell lysate (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40623 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 ...read more
Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBX5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HP1 alpha Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 150 and the C-terminus (residue 191) of human Chromobox Homolog 5 using the numbering given in entry NP_036249.1 (GeneID 23468).
Marker
Heterochromatin Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CBX5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:200-1:10000
  • Immunohistochemistry-Paraffin 1:200-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:10000
Application Notes
Chip, IP reactivity reported in (PMID: 23045645). Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Control
HP1 alpha Overexpression Lysate
Publications
Read Publications using
NB110-40623 in the following applications:

  • 3 publications
  • IP
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HP1 alpha Antibody

  • Antigen p25
  • chromobox homolog 5 (Drosophila HP1 alpha)
  • chromobox homolog 5 (HP1 alpha homolog, Drosophila)
  • chromobox homolog 5
  • chromobox protein homolog 5
  • Heterochromatin protein 1 homolog alpha
  • heterochromatin protein 1-alpha
  • HP1 alpha homolog
  • HP1 alpha
  • HP1
  • HP1A
  • HP1-ALPHA
  • HP1Hs alpha
  • HP1Hs-alpha

Background

CBX5 (chromobox protein homolog 5), also known as HP1 (heterochromatin protein 1 homolog alpha), is one of 3 isoforms of HP1 that binds heterochromatin and functions as a structural adaptor to stabilize and organize macromolecular complexes with nucleosomes for higher chromatin structure. All of the HP1 isoforms possess a chromodomain and chromoshadow domain and associate with methylated histones that mediate transcriptional repression. Alternate names for CBX5 include HP1 alpha, antigen p25, HP1Hs-alpha, and HP1A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HP1 alpha Antibody (NB110-40623)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for HP1 alpha Antibody (NB110-40623). (Showing 1 - 1 of 1 FAQs).

  1. I want to use the hp1 alpha antibody in zebrafish, and I think NB110-40623 might work. The online information of this product mentions that it recognize the region between residue 150 and the C-terminus (residue 191) of human Chromobox Homolog 5. Here is my question, do you use this 42 amino acid peptide as immunogen? If not, can you tell me the exact sequence of immunogen, or its identidy to zebrafish hp1 alpha whose access no. is AAI64295.1.
    • The immunogen for NB110-40623 was a synthetic peptide corresponding to the region 150-191, but the exact sequence can not be provided due to proprietary reasons. I did compare the homology between the human protein and the sequence you gave me, and it's greater than 95%.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CBX5
Entrez
Uniprot