HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free

Western Blot: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Heme oxygenase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Heme

Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human pancreas tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.

Flow Cytometry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Heme oxygenase 2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.

Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.

Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.

Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.

Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human breast tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.

Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human endometrium tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.

Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human Kidney tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.

Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human lymphoma tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.

Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human tonsil using anti-Heme oxygenase 2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI1C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free (NBP2-01407) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human HMOX2(NP_002125) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMOX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Theoretical MW: 35.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free

• heme oxygenase (decycling) 2
• heme oxygenase 2
• HMOX2
• HO2
• HO-2
• HO-2EC 1.14.99.3

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMOX2