HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free

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Western Blot: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Heme oxygenase 2 (Right lane) cDNA for 48 hrs and ...read more
Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human pancreas tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.
Flow Cytometry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Heme ...read more
Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.
Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.
Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.
Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.
Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human breast tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.
Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human endometrium tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.
Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human Kidney tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.
Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human lymphoma tissue using anti-Heme oxygenase 2 mouse monoclonal antibody.
Immunohistochemistry: HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free [NBP2-70895] - Staining of paraffin-embedded Human tonsil using anti-Heme oxygenase 2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI1C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human HMOX2(NP_002125) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HMOX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:500
Theoretical MW
35.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for HO-2/HMOX2 Antibody (OTI1C2) - Azide and BSA Free

  • heme oxygenase (decycling) 2
  • heme oxygenase 2
  • HMOX2
  • HO2
  • HO-2
  • HO-2EC 1.14.99.3

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HMOX2