HNRPGT Antibody (S05-2H3)

Western Blot: HNRPGT Antibody (S05-2H3) [NBP3-19655] - Western blot detection of HNRPGT in K562, Rat Brain, C6, 3T3, Hela cell lysates using NBP3-19655 (1:1000 diluted). Predicted band size: 43kDa. Observed band size: 43kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S05-2H3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

HNRPGT Antibody (S05-2H3) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human HNRPGT (Uniprot # O75526)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RBMXL2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for HNRPGT Antibody (S05-2H3)

• hnRNP G-T
• HNRNPG-T
• HNRNPGTRNA-binding motif protein, X-linked-like-2
• HNRPGTheterogeneous nuclear ribonucleoprotein G T
• RNA binding motif protein, X-linked-like 2
• testes specific heterogenous nuclear ribonucleoprotein G T
• testes-specific heterogenous nuclear ribonucleoprotein G-T
• Testis-specific heterogeneous nuclear ribonucleoprotein G-T

Background

HNRNPG belongs to the HNRPG subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two RRM domains that bind RNAs. This gene is intronless and is thought to be derived from a processed retroposon. However, unlike many retroposon-derived genes, this gene is not a pseudogene. The encoded protein has similarity to HNRPG and RBMY proteins and it is suggested to replace HNRPG protein function during meiotic prophase or act as a germ cell-specific splicing regulator. It primarily localizes to the nuclei of meiotic spermatocytes. This gene is a candidate for autosomal male infertility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBMXL2