hnRNP M Antibody (S06-3B1)

Western Blot: hnRNP M Antibody (S06-3B1) [NBP3-15042] - Western blot detection of hnRNP M in K562, C6, 3T3, Hela cell lysates using hnRNP M Rabbit mAb (1:1000 diluted). Predicted band size: 78kDa. Observed band size: 78kDa.

Immunohistochemistry-Paraffin: hnRNP M Antibody (S06-3B1) [NBP3-15042] - Immunohistochemistry of hnRNP M in paraffin-embedded Human tonsil using hnRNP M Rabbit mAb at dilution 1/50

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S06-3B1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

hnRNP M Antibody (S06-3B1) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human hnRNP M (Uniprot#: P52272)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HNRNPM
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for hnRNP M Antibody (S06-3B1)

• DKFZp547H118
• heterogeneous nuclear ribonucleoprotein M
• hnRNP M
• HNRNPM4
• HNRPM4heterogenous nuclear ribonucleoprotein M
• HNRPMheterogenous nuclear ribonucleoprotein M4
• HTGR1
• M4 protein
• N-acetylglucosamine receptor 1
• NAGR1hnRNA-binding protein M4

Background

Proteins that directly bind to nascent RNA polymerase II transcripts--the heterogenous nuclear ribonucleoproteins (hnRNPs)--play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64kDa subunit of cleavage stimulation factor, which is involved in end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPM