HNF1 Antibody

Product Discontinued

HNF1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human TCF-1.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNF1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of TCF-1 (F26) pAb in extracts from HUVEC cells.
  Immunohistochemistry (IHC) analyzes of TCF-1 (F26) pAb in paraffin-embedded human colon carcinoma tissue.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HNF1 Overexpression Lysate

Reactivity Notes

This antibody is reactive against Human, Mouse,.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HNF1 Antibody

• HNF1 homeobox A
• IDDM20
• LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor
• MODY3
• TCF1hepatic
• Transcription factor 1

Background

T-cell factor-1 (TCF-1) is a DNA-binding transcriptional activator that isessential for lymphoid cell development. The TCF family of transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. Several alternative splice variants of TCF-1 have been identified, including TCF-1A, which share a conserved amino terminus and differ in the carboxy terminal sequences. The Wnt mediated signaling pathway induces cytosolic beta-catenin binding to TCF proteins within the nucleus, leading to the enhanced expression of the Wnt target genes. The beta-catenin-TCF complexes are negatively regulated by the adenomatous polyposis coli (APC) tumor suppressor protein, which phosphorylates beta-catenin and, in turn, increases the degradation of cytosolic beta-catenin and inhibits the transcriptional activity of the TCF proteins. Mutations in the APC gene, which are commonly observed in colorectal carcinomas, disrupt this regulatory pathway and correlate with an accumulation of beta-catenin and the increased activation of the TCF target genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNF1A
• Entrez:
  • 6927(Human)
  • 21405(Mouse)