HNF-6/ONECUT1 Antibody (OTI4F12) - Azide and BSA Free

Western Blot: HNF-6/ONECUT1 Antibody (OTI4F12) - Azide and BSA Free [NBP2-70906] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF6.

Immunocytochemistry/Immunofluorescence: HNF-6/ONECUT1 Antibody (OTI4F12) - Azide and BSA Free [NBP2-70906] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HNF6.

Immunohistochemistry: HNF-6/ONECUT1 Antibody (OTI4F12) - Azide and BSA Free [NBP2-70906] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HNF6 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI4F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HNF-6/ONECUT1 Antibody (OTI4F12) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 85-198 of human HNF6 (NP_004489) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ONECUT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Theoretical MW: 50.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HNF-6/ONECUT1 Antibody (OTI4F12) - Azide and BSA Free

• HNF6
• HNF-6
• HNF-6A
• HNF6Ahepatocyte nuclear factor 6, alpha
• HNF6hepatocyte nuclear factor 6
• HNF-6One cut domain family member 1
• OC-1
• one cut homeobox 1one cut domain, family member 1
• ONECUT1

Background

Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ONECUT1