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HNF-3 alpha/FoxA1 Antibody (JF10-02)

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Western Blot: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Analysis of FOXA1 on different lysates using anti-FOXA1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: Mouse lung
Western Blot: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Analysis of FOXA1 on different lysates using anti-FOXA1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: Mouse lung
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Staining FOXA1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Analysis of paraffin-embedded rat prostate tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Analysis of paraffin-embedded human breast tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Analysis of paraffin-embedded mouse colon tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
JF10-02
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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HNF-3 alpha/FoxA1 Antibody (JF10-02) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within human HNF-3 alpha/FoxA1 aa 340-472. (SwissProt: P55317 Human;SwissProt: P35582 Mouse;SwissProt: P23512 Rat)
Localization
Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FOXA1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for HNF-3 alpha/FoxA1 Antibody (JF10-02)

  • forkhead box A1
  • Forkhead box protein A1
  • FoxA1
  • hepatocyte nuclear factor 3, alpha
  • hepatocyte nuclear factor 3-alpha
  • HNF3 alpha
  • HNF-3 alpha
  • HNF-3A
  • HNF-3-alpha
  • HNF3ATCF-3A
  • MGC33105
  • TCF3A
  • Transcription factor 3A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. Specific bands were detected for GATA‑3 full length (FL) at approximately 62 kDa and the splice form (SF) at approximately 52 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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HNF-3 alpha/FoxA1
Antibody (JF10-02)
NBP2-66889
HNF-3 alpha/FoxA1 Antibody (JF10-02)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol FOXA1