HNF-3 alpha/FoxA1 Antibody (JF10-02)

Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Staining FOXA1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western Blot: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -Analysis of FOXA1 on different lysates with Rabbit anti-FOXA1 antibody at 1/2,000 dilution.Lane 1: MCF7 cell lysateLane 2: HepG2 cell lysateLane 3: A549 cell lysateLane 4: LNCaP cell lysateLysates/proteins at 20 ug/Lane.Predicted band size: 49 kDaObserved band size: 49 kDaExposure time: 5 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Analysis of LNCaP cells labeling FOXA1 with Rabbit anti-FOXA1 antibody at 1/250 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FOXA1 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -Human breast carcinoma tissue with Rabbit anti-FOXA1 antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Human prostate tissue with Rabbit anti-FOXA1 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -Human prostate carcinoma tissue with Rabbit anti-FOXA1 antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -Mouse liver tissue with Rabbit anti-FOXA1 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -Rat liver tissue with Rabbit anti-FOXA1 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

HNF-3 alpha/FoxA1 Antibody (JF10-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human HNF-3 alpha/FoxA1 aa 320-472 / 472. (SwissProt: P55317 Human; SwissProt: P35582 Mouse; SwissProt: P23512 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FOXA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence IF-Cell-1:50-1:250, IF-Tissue-1:50-1:1,000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:2,000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HNF-3 alpha/FoxA1 Antibody (JF10-02)

• forkhead box A1
• Forkhead box protein A1
• FoxA1
• hepatocyte nuclear factor 3, alpha
• hepatocyte nuclear factor 3-alpha
• HNF3 alpha
• HNF-3 alpha
• HNF-3A
• HNF-3-alpha
• HNF3ATCF-3A
• MGC33105
• TCF3A
• Transcription factor 3A

Background

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXA1