HNF-3 alpha/FoxA1 Antibody (JF10-02)

Images

 
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Staining FOXA1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Western Blot: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -Analysis of FOXA1 on different lysates with Rabbit anti-FOXA1 antibody at 1/2,000 dilution.Lane 1: MCF7 cell lysateLane 2: HepG2 cell lysateLane 3: A549 ...read more
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Analysis of LNCaP cells labeling FOXA1 with Rabbit anti-FOXA1 antibody at 1/250 dilution.Cells were fixed in 4% ...read more
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -Human breast carcinoma tissue with Rabbit anti-FOXA1 antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen ...read more
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] - Human prostate tissue with Rabbit anti-FOXA1 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen ...read more
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -Human prostate carcinoma tissue with Rabbit anti-FOXA1 antibody at 1/1,000 dilution.The section was pre-treated using heat mediated ...read more
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -Mouse liver tissue with Rabbit anti-FOXA1 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval ...read more
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (JF10-02) [NBP2-66889] -Rat liver tissue with Rabbit anti-FOXA1 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-66889
    • Availability
      Product Discontinued

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HNF-3 alpha/FoxA1 Antibody (JF10-02) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within Human HNF-3 alpha/FoxA1 aa 320-472 / 472. (SwissProt: P55317 Human; SwissProt: P35582 Mouse; SwissProt: P23512 Rat)
Localization
Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FOXA1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence IF-Cell-1:50-1:250, IF-Tissue-1:50-1:1,000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:2,000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for HNF-3 alpha/FoxA1 Antibody (JF10-02)

  • forkhead box A1
  • Forkhead box protein A1
  • FoxA1
  • hepatocyte nuclear factor 3, alpha
  • hepatocyte nuclear factor 3-alpha
  • HNF3 alpha
  • HNF-3 alpha
  • HNF-3A
  • HNF-3-alpha
  • HNF3ATCF-3A
  • MGC33105
  • TCF3A
  • Transcription factor 3A

Background

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FOXA1