HMGB3/HMG4 Antibody (546519)

Western blot shows lysates of human placenta tissue. PVDF Membrane was probed with 2 µg/mL of Human/Mouse HMGB3 Monoclonal Antibody (Catalog # MAB55071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HMGB3 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HMGB3 was detected in immersion fixed Detroit 551 human skin fibroblast cell line using Human/Mouse HMGB3 Monoclonal Antibody (Catalog # MAB55071) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: 546519
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HMGB3/HMG4 Antibody (546519) Summary

• Immunogen: E. coli-derived recombinant human HMGB3
  Met1-Glu180
  Accession # O15347
• Specificity: Detects human and mouse HMGB3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human HMG-1, A1A, A1B, or A2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMGB3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HMGB3/HMG4 Antibody (546519)

• High mobility group protein 2a
• High mobility group protein 4
• high mobility group protein B3
• high-mobility group (nonhistone chromosomal) protein 4
• high-mobility group box 3
• HMG2A
• HMG-2a
• HMG2AHMG-4
• HMG4
• HMG4MGC90319
• HMGB3
• non-histone chromosomal protein

Background

High mobility group protein B3 (HMGB3; also HMG-4 and HMG-2a) is a 23 kDa nuclear protein and member of the HMGB family.  Human HMGB3 is synthesized as a 200 amino acid (aa) precursor, which is demethylated to produce the 199 aa mature chain.  The protein contains two HMG box DNA-binding domains (aa 9‑79 and 93‑161) and an acidic Asp/Glu-rich region (aa 181‑200).  Human HMGB3 shares 98% aa sequence identity with mouse and bovine HMGB3.  HMGB3 is expressed predominantly in the placenta.  It binds preferentially single-stranded DNA and unwinds double stranded DNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGB3
• Uniprot:
  • O15347()