Recombinant Mouse HMGB1/HMG-1 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA, IA
Concentration
0.5 mg/ml

Order Details

Recombinant Mouse HMGB1/HMG-1 Protein Summary

Description
Recombinant protein corresponding to Met1-Glu215 of mouse HMGB-1. Accession #: NM_010439.

Source: E.coli

Details of Functionality
The ED50 of this protein, as measured by mouse TNFa induction in RAW264.7 cells, is 3-25 ug/ml in the presence of 10 ug/ml Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
HMGB1
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Application Notes
The recombinant mouse HMGB-1 has been tested by bioassay, yielding ED50 of 3 ug/mL.
Theoretical MW
27.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid; 20 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 1 mM DTT.
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Mouse HMGB1/HMG-1 Protein

  • Amphoterin
  • high mobility group box 1
  • High mobility group protein 1
  • high mobility group protein B1
  • high-mobility group (nonhistone chromosomal) protein 1
  • high-mobility group box 1
  • HMG1
  • HMG-1
  • HMG1DKFZp686A04236
  • HMG3
  • HMGB1
  • SBP-1
  • Sulfoglucuronyl carbohydrate binding protein

Background

High-mobility group box-1 (HMGB1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79 and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol HMGB1
Entrez