HMGB1/HMG-1 Antibody (J2E1) - BSA Free

Western Blot: HMGB1/HMG-1 Antibody (J2E1) [NBP1-07087] - Cell lysates of Jurkat and HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HMGB1 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (J2E1) [NBP1-07087] - Paraffin embedded sections of human colon cancer tissue were incubated with anti-human HMGB1 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Product Discontinued

HMGB1/HMG-1 Antibody (J2E1) - BSA Free Summary

• Immunogen: Human HMGB1/HMG-1 (1-215aa) purified from Baculovirus.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMGB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:500-1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-300
  • Western Blot 1:500-2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HMGB1/HMG-1 Antibody (J2E1) - BSA Free

• Amphoterin
• high mobility group box 1
• High mobility group protein 1
• high mobility group protein B1
• high-mobility group (nonhistone chromosomal) protein 1
• high-mobility group box 1
• HMG1
• HMG-1
• HMG1DKFZp686A04236
• HMG3
• HMGB1
• SBP-1
• Sulfoglucuronyl carbohydrate binding protein

Background

High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Clone J2E1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HMGB1 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGB1
• Entrez:
  • 3146(Human)
• Uniprot:
  • P09429()