Human HLA G ELISA Kit (Colorimetric)

ELISA: Human HLA G ELISA Kit (Colorimetric) [NBP2-62174] - Example standard curve for Human HLA G ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: EDTA plasma, amniotic fluid, cell culture supernatant
• Standard Curve Range: 3.91 to 125U/ml
• Sensitivity: 0.6U/ml

Human HLA G ELISA Kit (Colorimetric) Summary

• Specificity: Detects soluble human HLA-G (leukocyte antigen-G) in EDTA plasma, amniotic fluid, and cell culture supernatant. Cross-reacts strongly with bovine, dog, goat, monkey, rabbit and sheep HLA-G. Cross-reacts weakly with cat HLA-G.
• Standard Curve Range: 3.91 to 125U/ml
• Sensitivity: 0.6U/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: Low Concentration %CV - 6.5% High - 7.4%
• Intra-Assay: Low Concentration %CV - 4.9% High - 3.5%
• Spike Recovery: Low 1 - 94.8%
  Low 2 - 88.2%
  Low 3 - 81.7%
  
  High 1 - 96.1%
  High 2 - 95.8%
  High 3 - 90.4%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HLA-G

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human HLA G ELISA Kit (Colorimetric)

• b2 microglobulin
• HLA class I histocompatibility antigen, alpha chain G
• HLA class I molecule
• HLA G antigen
• HLA G
• HLA-6.0
• HLA-G histocompatibility antigen, class I, G
• HLAG
• HLA-G
• major histocompatibility complex, class I, G
• MHC Class I Antigen G
• MHC class Ib antigen
• MHC-G
• mutant MHC class I antigen
• mutant MHC class Ib antigen
• sHLA-G
• Soluble HLA class I histocompatibility antigen, alpha chain G

Background

Human leukocyte antigen (HLA)-G is a non-classical major histocompatibility complex (MHC) class I gene belonging to the HLA class I family. The HLA class I family is subdivided into the classical (HLA-Ia) antigens comprised of HLA-A, -B, and -C molecules and the nonclassical (HLA-Ib) group including the HLA-E, -F, and -G molecules. Seven different splice variants of HLA-G have been observed including four membrane isoforms (HLA-G1, HLA-G2, HLA-G3, and HLA-G4) and three soluble isoforms (HLA-G5, HLA-G6 and HLA-G7). Proteolytic processing of HLA-G1 by metalloproteinases (MMPs) such as MMP-2 produces the soluble isoform, shedding HLA-G1. The HLA-G1 and HLA-G5 isoforms share a similar confirmation to the classical MHC class I protein, consisting of three extracellular domains including alpha1, alpha2, and alpha3 associated with Beta-2-microglobulin (B2M). Other HLA-G variants are shorter, missing one or two of the globular domains and are not bound to B2M. HLA-G receptors include KIR2DL4 (CD158d), with the highest affinity for ILT2 (LILRB1, CD85j) and ILT4 (LILRB2, CD85d) (1,2).

Discovered in cytotrophoblasts, HLA-G is involved in fetal maternal immune tolerance and some studies have linked its downregulation with severe preeclampsia (3). HLA-G mediated immune suppression works by impeding cell proliferation, differentiation, cytotoxicity, cytokine secretion and chemotaxis; and activation of regulatory cells and MDSCs or M2 type macrophage. HLA-G is constitutively expressed in immune-privileged sites such as pancreatic islets, mesenchymal stem cells (MSCs) and the cornea. Upregulation of HLA-G occurs in pathological states including cancer, allo-transplantations, viral infections, autoimmune and inflammatory diseases (4). In cancer, HLA-G expression is induced by hypoxia and has been correlated with advanced tumor stage, tumor metastasis, and poor therapeutic response and survival. HLA-G is an attractive tumor associated-antigen (TAA) for immunotherapy and is considered a major immune checkpoint (ICP).

References

1. Loustau, M., Anna, F., Drean, R., Lecomte, M., Langlade-Demoyen, P., & Caumartin, J. (2020). HLA-G Neo-Expression on Tumors. Front Immunol, 11:1685. PMID: 32922387

2. Lin A, Yan WH. (2018) Heterogeneity of HLA-G Expression in Cancers: Facing the Challenges. Front Immunol. 9:2164. PMID: 30319626

3. Djurisic S, Hviid TV. (2014) HLA Class Ib Molecules and Immune Cells in Pregnancy and Preeclampsia. Front Immunol. 5:652. PMID: 25566263

4. Lila N, Rouas-Freiss N, Dausset J, Carpentier A, Carosella ED. (2001) Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 98(21):12150-12155. PMID: 11572934

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for HLA G ELISA Kit (NBP2-62174). (Showing 1 - 1 of 1 FAQ).

1. Does this ELISA measure HLA-G1, HLA-G5, or both?
   • This Human HLA G ELISA Kit (NBP2-62174) measures shedded HLA-G1 and HLA-G5 in serum, plasma, amniotic fluid or cell culture supernatant

Bioinformatics

• Gene Symbol: HLA-G