Human HLA G ELISA Kit (Colorimetric)

ELISA: Human HLA G ELISA Kit (Colorimetric) [NBP2-62174] - Example standard curve for Human HLA G ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: EDTA plasma, amniotic fluid, cell culture supernatant
• Standard Curve Range: 3.91 to 125U/ml
• Sensitivity: 0.6U/ml

Human HLA G ELISA Kit (Colorimetric) Summary

• Specificity: Detects soluble human HLA-G (leukocyte antigen-G) in EDTA plasma, amniotic fluid, and cell culture supernatant. Cross-reacts strongly with bovine, dog, goat, monkey, rabbit and sheep HLA-G. Cross-reacts weakly with cat HLA-G.
• Standard Curve Range: 3.91 to 125U/ml
• Sensitivity: 0.6U/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: Low Concentration %CV - 6.5% High - 7.4%
• Intra-Assay: Low Concentration %CV - 4.9% High - 3.5%
• Spike Recovery: Low 1 - 94.8%
  Low 2 - 88.2%
  Low 3 - 81.7%
  
  High 1 - 96.1%
  High 2 - 95.8%
  High 3 - 90.4%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HLA-G

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human HLA G ELISA Kit (Colorimetric)

• b2 microglobulin
• HLA class I histocompatibility antigen, alpha chain G
• HLA class I molecule
• HLA G antigen
• HLA G
• HLA-6.0
• HLA-G histocompatibility antigen, class I, G
• HLAG
• HLA-G
• major histocompatibility complex, class I, G
• MHC Class I Antigen G
• MHC class Ib antigen
• MHC-G
• mutant MHC class I antigen
• mutant MHC class Ib antigen
• sHLA-G
• Soluble HLA class I histocompatibility antigen, alpha chain G

Background

Human leukocyte antigen (HLA)-G is a non-classical major histocompatibility complex (MHC) class I gene belonging to the HLA class I family. The HLA class I family is subdivided into the classical (HLA-Ia) antigens comprised of HLA-A, -B, and -C molecules and the nonclassical (HLA-Ib) group including the HLA-E, -F, and -G molecules. Seven different splice variants of HLA-G have been observed including four membrane isoforms (HLA-G1, HLA-G2, HLA-G3, and HLA-G4) and three soluble isoforms (HLA-G5, HLA-G6 and HLA-G7). Proteolytic processing of HLA-G1 by metalloproteinases (MMPs) such as MMP-2 produces the soluble isoform, shedding HLA-G1. The HLA-G1 and HLA-G5 isoforms share a similar confirmation to the classical MHC class I protein, consisting of three extracellular domains including alpha1, alpha2, and alpha3 associated with Beta-2-microglobulin (B2M). Other HLA-G variants are shorter, missing one or two of the globular domains and are not bound to B2M. HLA-G receptors include KIR2DL4 (CD158d), with the highest affinity for ILT2 (LILRB1, CD85j) and ILT4 (LILRB2, CD85d) (1,2).

Discovered in cytotrophoblasts, HLA-G is involved in fetal maternal immune tolerance and some studies have linked its downregulation with severe preeclampsia (3). HLA-G mediated immune suppression works by impeding cell proliferation, differentiation, cytotoxicity, cytokine secretion and chemotaxis; and activation of regulatory cells and MDSCs or M2 type macrophage. HLA-G is constitutively expressed in immune-privileged sites such as pancreatic islets, mesenchymal stem cells (MSCs) and the cornea. Upregulation of HLA-G occurs in pathological states including cancer, allo-transplantations, viral infections, autoimmune and inflammatory diseases (4). In cancer, HLA-G expression is induced by hypoxia and has been correlated with advanced tumor stage, tumor metastasis, and poor therapeutic response and survival. HLA-G is an attractive tumor associated-antigen (TAA) for immunotherapy and is considered a major immune checkpoint (ICP).

References

1. Loustau, M., Anna, F., Drean, R., Lecomte, M., Langlade-Demoyen, P., & Caumartin, J. (2020). HLA-G Neo-Expression on Tumors. Front Immunol, 11:1685. PMID: 32922387

2. Lin A, Yan WH. (2018) Heterogeneity of HLA-G Expression in Cancers: Facing the Challenges. Front Immunol. 9:2164. PMID: 30319626

3. Djurisic S, Hviid TV. (2014) HLA Class Ib Molecules and Immune Cells in Pregnancy and Preeclampsia. Front Immunol. 5:652. PMID: 25566263

4. Lila N, Rouas-Freiss N, Dausset J, Carpentier A, Carosella ED. (2001) Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 98(21):12150-12155. PMID: 11572934

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for HLA G ELISA Kit (NBP2-62174). (Showing 1 - 2 of 2 FAQ).

1. Does this ELISA measure HLA-G1, HLA-G5, or both?
   • This Human HLA G ELISA Kit (NBP2-62174) measures shedded HLA-G1 and HLA-G5 in serum, plasma, amniotic fluid or cell culture supernatant
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HLA-G