HLA G Antibody (87G) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
87G
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

Order Details

HLA G Antibody (87G) [Alexa Fluor® 700] Summary

Immunogen
721.221 cells transfected with full length HLA G cDNA with high surface HLA G Antibody (87G) expression. [UniProt# P17693]
Specificity
Membrane bound HLA G1 and soluble HLA G5.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLA-G
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Western Blot
Application Notes
The HLA G Antibody (87G) has been reported to work for Immunocytochemistry/Immunofluorescence and for blocking the interaction of HLA G with inhibitory receptors (see publications).
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HLA G Antibody (87G) [Alexa Fluor® 700]

  • b2 microglobulin
  • HLA class I histocompatibility antigen, alpha chain G
  • HLA class I molecule
  • HLA G antigen
  • HLA G
  • HLA-6.0
  • HLA-G histocompatibility antigen, class I, G
  • HLAG
  • HLA-G
  • major histocompatibility complex, class I, G
  • MHC Class I Antigen G
  • MHC class Ib antigen
  • MHC-G
  • mutant MHC class I antigen
  • mutant MHC class Ib antigen
  • sHLA-G
  • Soluble HLA class I histocompatibility antigen, alpha chain G

Background

Human leukocyte antigen (HLA)-G is a non-classical major histocompatibility complex (MHC) class I gene belonging to the HLA class I family. The HLA class I family is subdivided into the classical (HLA-Ia) antigens comprised of HLA-A, -B, and -C molecules and the nonclassical (HLA-Ib) group including the HLA-E, -F, and -G molecules. Seven different splice variants of HLA-G have been observed including four membrane isoforms (HLA-G1, HLA-G2, HLA-G3, and HLA-G4) and three soluble isoforms (HLA-G5, HLA-G6 and HLA-G7). Proteolytic processing of HLA-G1 by metalloproteinases (MMPs) such as MMP-2 produces the soluble isoform, shedding HLA-G1. The HLA-G1 and HLA-G5 isoforms share a similar confirmation to the classical MHC class I protein, consisting of three extracellular domains including alpha1, alpha2, and alpha3 associated with Beta-2-microglobulin (B2M). Other HLA-G variants are shorter, missing one or two of the globular domains and are not bound to B2M. HLA-G receptors include KIR2DL4 (CD158d), with the highest affinity for ILT2 (LILRB1, CD85j) and ILT4 (LILRB2, CD85d) (1,2).

Discovered in cytotrophoblasts, HLA-G is involved in fetal maternal immune tolerance and some studies have linked its downregulation with severe preeclampsia (3). HLA-G mediated immune suppression works by impeding cell proliferation, differentiation, cytotoxicity, cytokine secretion and chemotaxis; and activation of regulatory cells and MDSCs or M2 type macrophage. HLA-G is constitutively expressed in immune-privileged sites such as pancreatic islets, mesenchymal stem cells (MSCs) and the cornea. Upregulation of HLA-G occurs in pathological states including cancer, allo-transplantations, viral infections, autoimmune and inflammatory diseases (4). In cancer, HLA-G expression is induced by hypoxia and has been correlated with advanced tumor stage, tumor metastasis, and poor therapeutic response and survival. HLA-G is an attractive tumor associated-antigen (TAA) for immunotherapy and is considered a major immune checkpoint (ICP).

References

1. Loustau, M., Anna, F., Drean, R., Lecomte, M., Langlade-Demoyen, P., & Caumartin, J. (2020). HLA-G Neo-Expression on Tumors. Front Immunol, 11:1685. PMID: 32922387

2. Lin A, Yan WH. (2018) Heterogeneity of HLA-G Expression in Cancers: Facing the Challenges. Front Immunol. 9:2164. PMID: 30319626

3. Djurisic S, Hviid TV. (2014) HLA Class Ib Molecules and Immune Cells in Pregnancy and Preeclampsia. Front Immunol. 5:652. PMID: 25566263

4. Lila N, Rouas-Freiss N, Dausset J, Carpentier A, Carosella ED. (2001) Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 98(21):12150-12155. PMID: 11572934

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLA G Antibody (NBP1-43123AF700) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HLA G Antibody (NBP1-43123AF700) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-43123AF350
Alexa Fluor 405 NBP1-43123AF405
Alexa Fluor 488 NBP1-43123AF488
Alexa Fluor 532 NBP1-43123AF532
Alexa Fluor 594 NBP1-43123AF594
Alexa Fluor 647 NBP1-43123AF647
Alexa Fluor 700 NBP1-43123AF700
Alexa Fluor 750 NBP1-43123AF750
Allophycocyanin NBP1-43123APC
Allophycocyanin/Cy7 NBP1-43123APCCY7
Biotin NBP1-43123B
DyLight 350 NBP1-43123UV
DyLight 405 NBP1-43123V
DyLight 488 NBP1-43123G
DyLight 550 NBP1-43123R
DyLight 594 NBP1-43123DL594
DyLight 650 NBP1-43123C
DyLight 680 NBP1-43123FR
DyLight 755 NBP1-43123IR
HRP NBP1-43123H
Janelia Fluor 549 NBP1-43123JF549
Janelia Fluor 646 NBP1-43123JF646
PE NBP1-43123PE
PE/Atto594 NBP1-43123PEATT594
PE/Cy5.5 NBP1-43123PECY55
PE/Cy7 NBP1-43123PECY7
PerCP NBP1-43123PCP

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Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-G