HLA-E Antibody (3D12HLA-E) [PE]

Images

 
Flow Cytometry: HLA-E Antibody (3D12HLA-E) [PE] [NBP2-00277] - Staining of normal human peripheral blood cells with Anti-Human HLA-ABC FITC and Mouse IgG1 kappa Isotype Control PE (left) or Anti-Human HLA-E PE (right). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
3D12HLA-E
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

Order Details

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HLA-E Antibody (3D12HLA-E) [PE] Summary

Immunogen
The immunogen for this antibody was HLAE.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLA-E
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This 3D12 (HLA-E) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 ul (0.5 ug)/per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for HLA-E Antibody (3D12HLA-E) [PE]

  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • QA1

Background

The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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HLA-E NBP2-00277

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Bioinformatics

Gene Symbol HLA-E