HLA-E Antibody (1079755) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: IHC
• Clone: 1079755
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

HLA-E Antibody (1079755) [Alexa Fluor® 405] Summary

• Immunogen: HLA-E containing synthetic peptide
  Accession # P13747
• Specificity: Detects a synthetic peptide around aa 80 in Direct ELISA
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HLA-E Antibody (1079755) [Alexa Fluor® 405]

• DKFZp686P19218
• EA1.2
• EA2.1
• HLA class I histocompatibility antigen, alpha chain E
• HLA class I histocompatibility antigen, E alpha chain
• HLA-6.2
• HLAE
• HLA-E
• lymphocyte antigen
• major histocompatibility complex, class I, E
• MHC class I antigen E
• MHC HLA-E alpha-1
• MHC HLA-E alpha-2.1
• MHC
• QA1

Background

HLA-E is a class I histocompatibility antigen involved in immune self-nonself discrimination. HLA-E classically binds epitopes from HLA-A, HLA-B, HLA-C and HLA-G signal peptides and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors on NK and t-cell subsets.  HLA-E down-regulates NK cell cytolytic activity when presented on the cell surface.  It can also restrict non-canonical CD8+ T cells during natural infection with pathogens. HLA-E protein expression in normal human nonlymphoid organs is restricted to endothelial cells.  It is also basally expressed by B and T-lymphocytes, NK cells and macrophages.  TNF-alpha, IL-1beta and IFN-gamma up-regulated cell-surface expression of HLA-EE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.