HLA-E Antibody (1079755) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Clone: 1079755
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

HLA-E Antibody (1079755) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects a synthetic peptide around aa 80 in Direct ELISA
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HLA-E Antibody (1079755) [Alexa Fluor™ Plus 594]

• DKFZp686P19218
• EA1.2
• EA2.1
• HLA class I histocompatibility antigen, alpha chain E
• HLA class I histocompatibility antigen, E alpha chain
• HLA-6.2
• HLAE
• HLA-E
• lymphocyte antigen
• major histocompatibility complex, class I, E
• MHC class I antigen E
• MHC HLA-E alpha-1
• MHC HLA-E alpha-2.1
• MHC
• QA1

Background

HLA-E is a class I histocompatibility antigen involved in immune self-nonself discrimination. HLA-E classically binds epitopes from HLA-A, HLA-B, HLA-C and HLA-G signal peptides and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors on NK and t-cell subsets.  HLA-E down-regulates NK cell cytolytic activity when presented on the cell surface.  It can also restrict non-canonical CD8+ T cells during natural infection with pathogens. HLA-E protein expression in normal human nonlymphoid organs is restricted to endothelial cells.  It is also basally expressed by B and T-lymphocytes, NK cells and macrophages.  TNF-alpha, IL-1beta and IFN-gamma up-regulated cell-surface expression of HLA-EE.

1. Sharpe HR, Bowyer G, Brackenridge S, Lambe T. HLA-E: exploiting pathogen-host interactions for vaccine development. Clin Exp Immunol. 2019 May;196(2):167-177. doi: 10.1111/cei.13292. Epub 2019 Apr 9. PMID: 30968409; PMCID: PMC6468186. 
2. Lin Z, Bashirova AA, Viard M, Garner L, Quastel M, Beiersdorfer M, Kasprzak WK, Akdag M, Yuki Y, Ojeda P, Das S, Andresson T, Naranbhai V, Horowitz A, McMichael AJ, Hoelzemer A, Gillespie GM, Garcia-Beltran WF, Carrington M. HLA class I signal peptide polymorphism determines the level of CD94/NKG2-HLA-E-mediated regulation of effector cell responses. Nat Immunol. 2023 Jul;24(7):1087-1097. doi: 10.1038/s41590-023-01523-z. Epub 2023 Jun 1. PMID: 37264229; PMCID: PMC10690437. 
3. Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B. Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Blood. 2007 Apr 1;109(7):2806-14. doi: 10.1182/blood-2006-06-030213. PMID: 17179229.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.