HLA DRA Antibody - BSA Free

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP2-38619] - Staining of human colon.

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP2-38619] - Staining of human liver shows positivity in Kupffer cells.

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP2-38619] - Staining of human lymph node shows membranous positivity in lymphoid cells.

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP2-38619] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP2-38619] - Staining of human small intestine shows membranous positivity in lymphoid cells.

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP2-38619] - Staining in human lymph node and skeletal muscle tissues . Corresponding HLA-DRA RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP2-38619] - Staining of human cerebral cortex, colon, kidney and lymph node using Anti-HLA-DRA antibody NBP2-38619 (A) shows similar protein distribution across tissues to independent antibody NBP2-38691 (B).

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP2-38619] - Staining of human kidney.

Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP2-38619] - Staining of human cerebral cortex.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HLA DRA Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: LEIMTKRSNYTPITNVPPEVTVLTNS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HLA-DRA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  HLA DRA Protein (NBP2-38619PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HLA DRA Antibody - BSA Free

• FLJ51114
• histocompatibility antigen HLA-DR alpha
• HLA class II histocompatibility antigen, DR alpha chain
• HLA-DRA1
• major histocompatibility complex, class II, DR alpha
• MHC cell surface glycoprotein
• MHC class II antigen DRA
• MLRW

Background

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DRA
• Uniprot:
  • P01903()