HLA Class I Antibody (W6/32) - Low Endotoxin

Separation of leukocytes stained using anti-HLA Class I (W6/32) purified antibody (concentration in sample 4 ug/ml, GAM APC, red-filled) from leukocytes unstained by primary antibody (GAM APC, black-dashed) in Analysis (surface staining).

• Reactivity: Hu, Bv, Fe, Pm
• Applications: WB, ELISA, Flow, Func, ICC/IF, IHC, IP, CyTOF-ready
• Clone: W6/32
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Low Endotoxin
• Concentration: 1 mg/ml

HLA Class I Antibody (W6/32) - Low Endotoxin Summary

• Immunogen: Membrane of human tonsil cells
• Specificity: The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-A
• Purity: Protein A purified
• Endotoxin Note: Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

This antibody does not react with: Rabbit

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.2 um filter sterilized
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HLA Class I Antibody (W6/32) - Low Endotoxin

• A-10 alpha chain
• FLJ26655
• HLA class I histocompatibility antigen, A-1 alpha chain
• HLA class I histocompatibility antigen, A-28 alpha chain
• HLA class I histocompatibility antigen, A-9 alpha chain
• HLA Class I
• HLAA
• HLA-A
• HLA-ABC
• major histocompatibility complex, class I, A
• MHC class I antigen A*1
• MHC class I antigen A*11
• MHC class I antigen A*80

Background

HLA CLass I is involved in the presentation of foreign antigens to the immune system. This antibody is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody is also suitable as a positive control for HLA tissue typing and crossmatching.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-A