HLA B7 Antibody (BB7.1) - BSA Free

Flow Cytometry: HLA B7 Antibody (BB7.1) [NB100-64159] - Using the PE direct conjugate A surface stain was performed on human peripheral blood lymphocytes with HLA B7 (BB7.1) antibody NB100-64159PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Flow Cytometry: HLA B7 Antibody (BB7.1) [NB100-64159] - Staining of human peripheal blood lymphocytes from an HLA-B7 positive donor with mouse anti human HLA-B7

Flow Cytometry: HLA B7 Antibody (BB7.1) [NB100-64159] - Analysis using the FITC conjugate of NB100-64159. Staining of human peripheral blood lymphocytes.

Flow Cytometry: HLA B7 Antibody (BB7.1) [NB100-64159] - A surface stain was performed on human peripheral blood lymphocytes with HLA B7 (BB7.1) antibody NB100-64159PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature.

Flow Cytometry: HLA B7 Antibody (BB7.1) [NB100-64159] - Using the PE direct conjugate A surface stain was performed on human peripheral blood monocytes with HLA B7 (BB7.1) antibody NB100-64159PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

• Reactivity: Hu
• Applications: Flow
• Clone: BB7.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HLA B7 Antibody (BB7.1) - BSA Free Summary

• Immunogen: Papain solubilized HLA-B7 antigen
• Specificity: Recognizes the HLA-B7 antigen and does not cross-react with HLA-B27 or other related antigens. It can be used to distinguish true HLA-B27 positives from false HLA-B27 positives (i.e. HLA-B7 positive) in the investigation of diseases such as ankylosing spondylitis and anterior uveitis.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Has been used successfully in immunohistochemistry on paraffin sections reported by a customer review.

Reactivity Notes

Predicted cross-reactivities: Cynomolgus monkey

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for HLA B7 Antibody (BB7.1) - BSA Free

• ankylosing spondylitis
• AS
• b'DT
• Bw-22
• Bw-4
• Bw-41
• Bw-44
• Bw-45
• Bw-46
• Bw-47
• Bw-48
• Bw-50
• Bw-52
• Bw-53
• Bw-54
• Bw-55
• Bw-56
• Bw-57
• Bw-58
• Bw-60
• HLA class I histocompatibility antigen, B alpha chain
• HLA class I histocompatibility antigen, B-12 alpha chain
• HLA class I histocompatibility antigen, B-21 alpha chain
• HLA class I histocompatibility antigen, B-5 alpha chain
• HLA class I histocompatibility antigen, B-7 alpha chain
• HLAB
• HLA-B27
• HLA-B73
• leukocyte antigen class I-B
• lymphocyte antigen
• major histocompatibility complex, class I, B
• MGC111087
• MHC class I antigen B*13
• MHC class I antigen B*14
• MHC class I antigen B*15
• MHC class I antigen B*18
• MHC class I antigen B*27
• MHC class I antigen B*35
• MHC class I antigen B*37
• MHC class I antigen B*38
• MHC class I antigen B*39
• MHC class I antigen B*40
• MHC class I antigen B*41
• MHC class I antigen B*42
• MHC class I antigen B*44
• MHC class I antigen B*45
• MHC class I antigen B*46
• MHC class I antigen B*47
• MHC class I antigen B*48
• MHC class I antigen B*49
• MHC class I antigen B*50
• MHC class I antigen B*51
• MHC class I antigen B*52
• MHC class I antigen B*53
• MHC class I antigen B*54
• MHC class I antigen B*55
• MHC class I antigen B*56
• MHC class I antigen B*57
• MHC class I antigen B*58
• MHC class I antigen B*59
• MHC class I antigen B*67
• MHC class I antigen B*7
• MHC class I antigen B*73
• MHC class I antigen B*78
• MHC class I antigen B*8
• MHC class I antigen B*81
• MHC class I antigen B*82
• MHC class I antigen GN00104
• MHC class I antigen HLA-B heavy chain
• MHC class I antigen SHCHA
• MHC Class I HLA heavy chain
• MHC HLA-B cell surface glycoprotein
• SPDA1

Background

HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa. Hundreds of HLA B alleles have been described. Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). This antibody can be used for HLA typing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA B7 Antibody (NB100-64159)(1)

Publication using NB100-64159

• Lulu AM, Cummings KL, Jeffery ED et al. Characteristics of Immune Memory and Effector Activity to Cancer-Expressed MHC Class I Phosphopeptides Differ in Healthy Donors and Ovarian Cancer Patients Cancer Immunology Research 2021-11-01 [PMID: 34413086] (Flow Cytometry)

FAQs for HLA B7 Antibody (NB100-64159). (Showing 1 - 1 of 1 FAQ).

1. We are interested in HLA-B7 antibodies that could be used for the immunohistochemistry of paraffin-embedded tissue. HLA B7 Antibody (BB7.1) offered in your company is described to fulfill those requirements (catalog # NB100-64159, 0.2mg). May I kindly ask you if you have a pictures of the performance of those antibodies for the IHC-P application? Unfortunately, we were quite unlucky with 3 HLA-B7 products and it seems to be not so easy to get a specific for HLA-B7 allele staining working for IHC-P. Therefore, we would like to ask if you can provide a test vial of NB100-64159? I haven't been testing BB7.1 clone of HLA-B7 antibodies from any other company. But I have the very last hope - may I ask you if you are planning to validate NB100-6415 BB7.1 clone for IHC-P? The only IHC-P applicable antibodies suggested by Biocompare are EP2624 antibodies from Abcam - those are unspecific (equally stain lymphocytes from HLA-B7pos and HLA-B7neg donors).
   • I looked into this product for you and actually have found out it has not been tested in IHC, which is why we had no images listed. So far this product has only been validated for flow cytometry. Have you ever used this clone from another company? If so, this most likely will not work as well since it is a licensed clone. Unfortunately we are unable to provide any free samples at this time per company policy. Unfortunately we do not have anything else to provide for this target at this time as the other two antibodies are just the directly conjugated version of this clone. A good place to check would be Biocompare as this site compares various products from different biology companies and you may be able to find something there. Since NB100-64159 can stain in a Flow application there is a good possibility it should also be effective in IHC, but since we cannot guarantee this I can direct you to our Innovators Reward Program. The great thing about this program is that you will be eligible to save money and receive a discount for testing a new application.

Bioinformatics

• Gene Symbol: HLA-B
• Uniprot:
  • P01889(Human)