HLA B Antibody - Azide and BSA Free

Western Blot: HLA B Antibody [H00003106-B01P] - Analysis of HLA-B expression in human spleen.

Western Blot: HLA B Antibody [H00003106-B01P] - Analysis of HLA-B expression in transfected 293T cell line by HLA-B polyclonal antibody. Lane 1: HLA-B transfected lysate(39.82 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HLA B Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: HLA-B (AAH13187.1, 1 a.a. - 362 a.a.) full-length human protein. MRVTAPRTVLLLLSGALALTETWAGSHSMRYFYTAMSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQICKTNTQTYRESLRNLRGYYNQSEAGSHTLQRMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAAREAEQLRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTIPIVGIVAGLAVLAVVVIGAVVATVMCRRKSSGGKGGSYSQAASSDSAQGSDVSLTA
• Specificity: HLA-B - major histocompatibility complex, class I, B,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: HLA-B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Western Blot
• Application Notes: Antibody reactivity against transfected lysate and tissue lysate for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HLA B Antibody - Azide and BSA Free

• ankylosing spondylitis
• AS
• B-4901
• b'DT
• Bw-22
• Bw-4
• Bw-41
• Bw-44
• Bw-45
• Bw-46
• Bw-47
• Bw-48
• Bw-50
• Bw-52
• Bw-53
• Bw-54
• Bw-55
• Bw-56
• Bw-57
• Bw-58
• Bw-60
• HLA class I histocompatibility antigen, B alpha chain
• HLA class I histocompatibility antigen, B-12 alpha chain
• HLA class I histocompatibility antigen, B-21 alpha chain
• HLA class I histocompatibility antigen, B-5 alpha chain
• HLA class I histocompatibility antigen, B-7 alpha chain
• HLAB
• HLA-B
• HLA-B27
• HLA-B73
• leukocyte antigen class I-B
• lymphocyte antigen
• major histocompatibility complex, class I, B
• MGC111087
• MHC class I antigen B*13
• MHC class I antigen B*14
• MHC class I antigen B*15
• MHC class I antigen B*18
• MHC class I antigen B*27
• MHC class I antigen B*35
• MHC class I antigen B*37
• MHC class I antigen B*38
• MHC class I antigen B*39
• MHC class I antigen B*40
• MHC class I antigen B*41
• MHC class I antigen B*42
• MHC class I antigen B*44
• MHC class I antigen B*45
• MHC class I antigen B*46
• MHC class I antigen B*47
• MHC class I antigen B*48
• MHC class I antigen B*49
• MHC class I antigen B*50
• MHC class I antigen B*51
• MHC class I antigen B*52
• MHC class I antigen B*53
• MHC class I antigen B*54
• MHC class I antigen B*55
• MHC class I antigen B*56
• MHC class I antigen B*57
• MHC class I antigen B*58
• MHC class I antigen B*59
• MHC class I antigen B*67
• MHC class I antigen B*7
• MHC class I antigen B*73
• MHC class I antigen B*78
• MHC class I antigen B*8
• MHC class I antigen B*81
• MHC class I antigen B*82
• MHC class I antigen GN00104
• MHC class I antigen HLA-B heavy chain
• MHC class I antigen SHCHA
• MHC Class I HLA heavy chain
• MHC HLA-B cell surface glycoprotein
• SPDA1

Background

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA B Antibody (H00003106-B01P)(3)

Publications using H00003106-B01P

• Lorente E, Infantes S, Abia D et al. A viral, transporter associated with antigen processing (TAP)-independent, high affinity ligand with alternative interactions endogenously presented by the nonclassical human leukocyte antigen E class I molecule. J Biol Chem. 2012-10-12 [PMID: 22927436]
• Lorente E, Infantes S, Barnea E et al. Multiple viral ligands naturally presented by different class I molecules in transporter antigen processing-deficient vaccinia virus-infected cells. J Virol. 2012-01-01 [PMID: 22031944]
• Lorente E, Garcia R, Lopez D. Allele-dependent Processing Pathways Generate the Endogenous Human Leukocyte Antigen (HLA) Class I Peptide Repertoire in Transporters Associated with Antigen Processing (TAP)-deficient Cells. J Biol Chem. 2011-09-13 [PMID: 21914809]

Bioinformatics

• Gene Symbol: HLA-B
• Entrez:
  • 3106(Human)
• Uniprot:
  • AAH13187.1(Human)