HLA B Antibody (JOAN-1)

Formalin-fixed, paraffin-embedded human lymph node stained with HLA B antibody (JOAN-1). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: Flow, ICC/IF, ICC/IF
• Clone: JOAN-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

HLA B Antibody (JOAN-1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11520)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Normal human peripheral blood lymphocytes (Uniprot: P01889)
• Localization: Cell Surface
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for HLA B Antibody (JOAN-1)

• ankylosing spondylitis
• AS
• B-4901
• b'DT
• Bw-22
• Bw-4
• Bw-41
• Bw-44
• Bw-45
• Bw-46
• Bw-47
• Bw-48
• Bw-50
• Bw-52
• Bw-53
• Bw-54
• Bw-55
• Bw-56
• Bw-57
• Bw-58
• Bw-60
• HLA class I histocompatibility antigen, B alpha chain
• HLA class I histocompatibility antigen, B-12 alpha chain
• HLA class I histocompatibility antigen, B-21 alpha chain
• HLA class I histocompatibility antigen, B-5 alpha chain
• HLA class I histocompatibility antigen, B-7 alpha chain
• HLAB
• HLA-B
• HLA-B27
• HLA-B73
• leukocyte antigen class I-B
• lymphocyte antigen
• major histocompatibility complex, class I, B
• MGC111087
• MHC class I antigen B*13
• MHC class I antigen B*14
• MHC class I antigen B*15
• MHC class I antigen B*18
• MHC class I antigen B*27
• MHC class I antigen B*35
• MHC class I antigen B*37
• MHC class I antigen B*38
• MHC class I antigen B*39
• MHC class I antigen B*40
• MHC class I antigen B*41
• MHC class I antigen B*42
• MHC class I antigen B*44
• MHC class I antigen B*45
• MHC class I antigen B*46
• MHC class I antigen B*47
• MHC class I antigen B*48
• MHC class I antigen B*49
• MHC class I antigen B*50
• MHC class I antigen B*51
• MHC class I antigen B*52
• MHC class I antigen B*53
• MHC class I antigen B*54
• MHC class I antigen B*55
• MHC class I antigen B*56
• MHC class I antigen B*57
• MHC class I antigen B*58
• MHC class I antigen B*59
• MHC class I antigen B*67
• MHC class I antigen B*7
• MHC class I antigen B*73
• MHC class I antigen B*78
• MHC class I antigen B*8
• MHC class I antigen B*81
• MHC class I antigen B*82
• MHC class I antigen GN00104
• MHC class I antigen HLA-B heavy chain
• MHC class I antigen SHCHA
• MHC Class I HLA heavy chain
• MHC HLA-B cell surface glycoprotein
• SPDA1

Background

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA B Antibody (NBP2-45000)(1)

Publication using NBP2-45000

• Taojun He, Yilin Deng, Fang Zhang, Jin Zhang, Luhong Zhu, Qinjin Wang, Jie Ning, Hui Wu, Hanmei Yuan, Bin Li, Chao Wu Characteristics of Norovirus capsid protein-specific CD8 + T-Cell responses in previously infected individuals Virulence 2024-05-27 [PMID: 38803081]

Bioinformatics

• Gene Symbol: HLA-B
• Uniprot:
  • P01889(Human)