HLA B Antibody (JOAN-1)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, ICC/IF
Clone
JOAN-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Catalog# & Formulation Size Price

HLA B Antibody (JOAN-1) Summary

Immunogen
Normal human peripheral blood lymphocytes (Uniprot: P01889)
Localization
Cell Surface
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLA-B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11520)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for HLA B Antibody (JOAN-1)

  • ankylosing spondylitis
  • AS
  • B-4901
  • b'DT
  • Bw-22
  • Bw-4
  • Bw-41
  • Bw-44
  • Bw-45
  • Bw-46
  • Bw-47
  • Bw-48
  • Bw-50
  • Bw-52
  • Bw-53
  • Bw-54
  • Bw-55
  • Bw-56
  • Bw-57
  • Bw-58
  • Bw-60
  • HLA class I histocompatibility antigen, B alpha chain
  • HLA class I histocompatibility antigen, B-12 alpha chain
  • HLA class I histocompatibility antigen, B-21 alpha chain
  • HLA class I histocompatibility antigen, B-5 alpha chain
  • HLA class I histocompatibility antigen, B-7 alpha chain
  • HLAB
  • HLA-B
  • HLA-B27
  • HLA-B73
  • leukocyte antigen class I-B
  • lymphocyte antigen
  • major histocompatibility complex, class I, B
  • MGC111087
  • MHC class I antigen B*13
  • MHC class I antigen B*14
  • MHC class I antigen B*15
  • MHC class I antigen B*18
  • MHC class I antigen B*27
  • MHC class I antigen B*35
  • MHC class I antigen B*37
  • MHC class I antigen B*38
  • MHC class I antigen B*39
  • MHC class I antigen B*40
  • MHC class I antigen B*41
  • MHC class I antigen B*42
  • MHC class I antigen B*44
  • MHC class I antigen B*45
  • MHC class I antigen B*46
  • MHC class I antigen B*47
  • MHC class I antigen B*48
  • MHC class I antigen B*49
  • MHC class I antigen B*50
  • MHC class I antigen B*51
  • MHC class I antigen B*52
  • MHC class I antigen B*53
  • MHC class I antigen B*54
  • MHC class I antigen B*55
  • MHC class I antigen B*56
  • MHC class I antigen B*57
  • MHC class I antigen B*58
  • MHC class I antigen B*59
  • MHC class I antigen B*67
  • MHC class I antigen B*7
  • MHC class I antigen B*73
  • MHC class I antigen B*78
  • MHC class I antigen B*8
  • MHC class I antigen B*81
  • MHC class I antigen B*82
  • MHC class I antigen GN00104
  • MHC class I antigen HLA-B heavy chain
  • MHC class I antigen SHCHA
  • MHC Class I HLA heavy chain
  • MHC HLA-B cell surface glycoprotein
  • SPDA1

Background

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HLA-B
Uniprot