HIV TAT specific factor 1 Antibody

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Western Blot: HIV TAT specific factor 1 Antibody [NBP1-06041] - (1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HIV TAT specific factor 1 Antibody Summary

Immunogen
Peptide with sequence C-QELYGDGKDGDTQTD corresponding to internal region (near N-Terminus) according to NP_055315.2.
Epitope
C-QELYGDGKDGDTQTD
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HTATSF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 140 kDa band observed in Daudi lysates is reported in scientific literature (PMID: 8849451). An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-06041.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HIV TAT specific factor 1 Antibody

  • cofactor required for Tat activation of HIV-1 transcription
  • dJ196E23.2
  • HIV TAT specific factor 1
  • HIV Tat-specific factor 1
  • HIV-1 Tat specific factor 1
  • TAT-SF1

Background

Whereas most DNA sequence-specific transcription factors increase the rate of initiation and interact with enhancer or promoter DNA, human immunodeficiency virus-1 (HIV-1) Tat predominantly stimulates elongation and interacts with the trans-acting responsive (TAR) RNA element. Tat is essential for HIV replication.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIV TAT specific factor 1 Antibody (NBP1-06041)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HTATSF1
Entrez
Uniprot