HIV-1 Tat specific factor 1 Antibody

Western Blot: HIV-1 Tat specific factor 1 Antibody [NBP1-06041] - (1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HIV-1 Tat specific factor 1 Antibody Summary

• Immunogen: Peptide with sequence C-QELYGDGKDGDTQTD corresponding to internal region of HIV-1 Tat specific factor 1 (near N-Terminus) according to NP_055315.2.
• Epitope: C-QELYGDGKDGDTQTD
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HTATSF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 140 kDa band observed in Daudi lysates is reported in scientific literature (PMID: 8849451). An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HIV-1 Tat specific factor 1 Antibody

• cofactor required for Tat activation of HIV-1 transcription
• dJ196E23.2
• HIV TAT specific factor 1
• HIV Tat-specific factor 1
• HIV-1 Tat specific factor 1
• TAT-SF1

Background

Whereas most DNA sequence-specific transcription factors increase the rate of initiation and interact with enhancer or promoter DNA, human immunodeficiency virus-1 (HIV-1) Tat predominantly stimulates elongation and interacts with the trans-acting responsive (TAR) RNA element. Tat is essential for HIV replication.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIV-1 Tat specific factor 1 Antibody (NBP1-06041)(1)

Publication using NBP1-06041

• Zhou M, Deng L, Lacoste V et al. Coordination of transcription factor phosphorylation and histone methylation by the P-TEFb kinase during human immunodeficiency virus type 1 transcription. J Virol 2004-12-01 [PMID: 15564463]

Bioinformatics

• Gene Symbol: HTATSF1
• Entrez:
  • 27336(Human)
  • 317612(Rat)
• Uniprot:
  • NP_055315.2(Human)