Western Blot: Histone H3 [Trimethyl Lys9] Antibody [NB21-1073] - WB analysis of H3K9me3 in C. elegans embryo lysate.
Immunocytochemistry: Histone H3 [Trimethyl Lys9] Antibody [NB21-1073] - Immunofluorescence: [NB21-1073] - Histone H3 K9me3 antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI(blue).
Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys9] Antibody [NB21-1073] - 2 ug of NB21-1073 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and ...read more
Western Blot: Histone H3 [Trimethyl Lys9] Antibody [NB21-1073] - Western blot analysis of Histone H3 K9me3 in HeLa histone preps.
Dot Blot: Histone H3 [Trimethyl Lys9] Antibody [NB21-1073] - Dot blot analysis of H3K9me3 in picomoles of peptide.
Histone H3 [Trimethyl Lys9] Antibody [NB21-1073]
Hu, Mu, Ce, Ye, Rt, Ch, Dr, Pl, XpSpecies Glossary
Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Yeast reactivity reported in scientific literature (PMID: 25582094)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.
Alternate Names for Histone H3 [Trimethyl Lys9] Antibody
H3 histone, family 3A
Transcriptional silencing of specific genes and repetitive elements is known to be regulated partially through methylation of histone H3 at lysine 9, a hallmark of constitutive heterochromatin. In placenta, OCT2 expression and its ultimate function as a cation transporter is linked with the abundance of H3K9me3; when this methylation of histone H3 occurs in excess, mRNA for OCT2 decreases. Centromeres also are distinctly affected by the H3K9me3 modification, in that it co-localizes with several centromeric proteins, and affects the DNA methylation, particularly at the periphery of centromeric DNA. Gene expression is silenced when there is coordination between the H3K9me3 and H3K4modifications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post.
Histone H3 Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me... Read full blog post.
EZH2: Epigenetic Regulation Made Easy! Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i... Read full blog post.
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