Histone H3 [Trimethyl Lys4] Antibody

Images

 
Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Western blot analysis of HeLa histone prep (A), NIH 3T3 histone prep (B), HeLa whole cell extract (C), and NIH 3T3 whole cell extract (D) using Histone H3 ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Histone H3 K4me3 antibody tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1023 and 20ul of magnetic IgG beads per ...read more
Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - WB analysis of Histone H3 K4me3 in C. elegans embryo nuclear extract.
Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - WB analysis of Histone H3 K4me3 in 1. HeLa histone prep and 2. NIH 3T3 histone prep lysates.
Dot Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Dot Blot analysis of Histone H3 K4me3 using the peptides listed above in 10, 3, and 1 picomoles of peptide.
Histone H3 [Trimethyl Lys4] Antibody [NB21-1023]

Product Details

Summary
Reactivity Hu, Mu, Ce, Rt, Ch, Dr, Pl, XpSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Histone H3 [Trimethyl Lys4] Antibody Summary

Immunogen
Synthetic trimethylated peptide surrounding Lysine 4 of human Histone H3.2 [Swiss Prot Q71DI3].
Modification
Trimethyl Lys4
Localization
Nucleus. Chromosome.
Predicted Species
Rat (100%), Chicken (100%), Drosophila (100%), Xenopus (100%), Plant (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H3C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This Histone H3 K4me3 antibody is useful for ChIP, Western Blot, Immunocytochemistry/Immunofluorescence, and Dot Blot. In Western Blot a band is seen ~15kDa in C. elegans embryo nuclear extract, HeLa histone prep, and NIH 3T3 histone prep lysates.
Positive Control
GAPDH primer (NBP1-71650)
RPL30 primer (NBP1-71651)
MYOD1 primer (NBP1-71652)
AFM primer (NBP1-71653)
alpha Satellite repeat primer (NBP1-71654)
Satellite2 repeat primer (NBP1-71655)
HeLa Lysate (NBP1-78155)
Control Peptide
Histone H3 Peptide (NB21-1023PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB21-1023 in the following applications:

Publications
Read Publication using NB21-1023.

Reactivity Notes

Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant (Nicotiana tabacum) based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H3 [Trimethyl Lys4] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • H3K4Me3
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

H3K4Me3 is a common epigenetic modification that occurs in close proximity to nearly 2/3 of human gene promoters, and seems to balance another modification, H3K27Me3. These two modifications work in tandem to differentiate and/or maintain stem cells (PMID: 18371363). Zygotic cells are activated in the most part by H3K4Me3 (PMID: 19758566); whereas, cancer is associated with disrupted associations between K3K4Me3 and ING1 (PMID: 18533182).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H3 Antibody (NB21-1023)(1)

Review for Histone H3 Antibody (NB21-1023) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB21-1023:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Histone H3 NB21-1023
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reviewed by:
Anonymous
WB Human 06/10/2014
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Summary

ApplicationWestern Blot
Sample TestedLysate from MCF 7 and HCT 116 cells
SpeciesHuman
CommentsWestern blot with whole cell lysate gives a non-specific band at around 28 KDa, but it is reduced in histone prepration

Blocking

Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:2000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:20K

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 30sec

Comments

CommentsWestern blot with whole cell lysate gives a non-specific band at around 28 KDa, but it is reduced in histone prepration

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB21-1023) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-1023AF405
Alexa Fluor 488 Labeled NB21-1023AF488
Alexa Fluor 647 Labeled NB21-1023AF647
Alexa Fluor 700 Labeled NB21-1023AF700
Biotin Labeled NB21-1023B
DyLight 405 Labeled NB21-1023V
DyLight 488 Labeled NB21-1023G
DyLight 550 Labeled NB21-1023R
DyLight 650 Labeled NB21-1023C
FITC Labeled NB21-1023F
HRP Labeled NB21-1023H

Additional Histone H3 Products

Bioinformatics Tool for Histone H3 Antibody (NB21-1023)

Discover related pathways, diseases and genes to Histone H3 Antibody (NB21-1023). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on Histone H3.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Recent Reviews

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Anonymous
06/10/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HIST2H3C
Entrez
Uniprot