Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Western blot analysis of HeLa histone prep (A), NIH 3T3 histone prep (B), HeLa whole cell extract (C), and NIH 3T3 whole cell extract (D) using Histone H3 ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Histone H3 K4me3 antibody tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1023 and 20ul of magnetic IgG beads per ...read more
Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - WB analysis of Histone H3 K4me3 in C. elegans embryo nuclear extract.
Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - WB analysis of Histone H3 K4me3 in 1. HeLa histone prep and 2. NIH 3T3 histone prep lysates.
Dot Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Dot Blot analysis of Histone H3 K4me3 using the peptides listed above in 10, 3, and 1 picomoles of peptide.
Hu, Mu, Ce, Chicken, Drosophila, Plant, Rat, XenopusSpecies Glossary
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 1 ug/ml
Chromatin Immunoprecipitation 2-5 ug per million cells
Dot Blot 1:1000
This Histone H3 K4me3 antibody is useful for ChIP, Western Blot, Immunocytochemistry/Immunofluorescence, and Dot Blot. In Western Blot a band is seen ~15kDa in C. elegans embryo nuclear extract, HeLa histone prep, and NIH 3T3 histone prep lysates.
Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant (Nicotiana tabacum) based on 100% sequence homology.
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.
Alternate Names for Histone H3 [Trimethyl Lys4] Antibody
H3 histone, family 3A
H3K4Me3 is a common epigenetic modification that occurs in close proximity to nearly 2/3 of human gene promoters, and seems to balance another modification, H3K27Me3. These two modifications work in tandem to differentiate and/or maintain stem cells (PMID: 18371363). Zygotic cells are activated in the most part by H3K4Me3 (PMID: 19758566); whereas, cancer is associated with disrupted associations between K3K4Me3 and ING1 (PMID: 18533182).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post.
Histone H3 Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me... Read full blog post.
EZH2: Epigenetic Regulation Made Easy! Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i... Read full blog post.