Histone H2AX Antibody - BSA Free

Western Blot: Histone H2AX Antibody [NB100-383] - Whole cell lysate (50 ug) from HEK293T cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: Histone H2AX Antibody [NB100-383] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX (NB100-383). Detection: DAB

Western Blot: Histone H2AX Antibody [NB100-383] - Detection of mouse H2AX by western blot. Samples: Whole cell lysate (50 ug) from F9 and L1210 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody A300-083A (lot A300-083A-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Simple Western: Histone H2AX Antibody [NB100-383] - Simple Western lane view shows a specific band for H2AX in 0.2 mg/ml of HEK lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.

Simple Western: Histone H2AX Antibody [NB100-383] - Electropherogram image(s) of corresponding Simple Western lane view. H2AX antibody was used at 10 ug/ml dilution on HEK lysate(s).

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Histone H2AX Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Histone H2AX Antibody - BSA Free (NB100-383) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western and IP. Anti-Histone H2AX Antibody: Cited in 14 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014).
• Specificity: This is specific for H2AX. It is not known if this also recognizes the phosphorylated form of H2AX.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H2AX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation
  • Simple Western 10 ug/ml
  • Western Blot 1:2000-1:10000
• Application Notes: By Western blot, this antibody recognizes a band at ~25 kDa, representing monoubiquinated H2AX protein and ~14 kDa, representing H2AX. IP, IHC reported in (PMID: 22426396). ICC/IF reported in (PMID: 20203688). br/>In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HEK lysate, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 28 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  Histone H2AX Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Histone H2AX Antibody - BSA Free

• H2A.X
• H2A/X
• H2AFX
• Histone H2AX

Background

Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone H2AX Antibody (NB100-383)(14)

Publications using NB100-383

• Kaima Tsukada, Samuel E Jones, Julius Bannister, Mary-Anne Durin, Iolanda Vendrell, Matthew Fawkes, Roman Fischer, Benedikt M Kessler, J Ross Chapman, Andrew N Blackford BLM and BRCA1-BARD1 coordinate complementary mechanisms of joint DNA molecule resolution. Molecular cell 2024-01-19 [PMID: 38266639]
• S Balasubram, M Andreani, JG Andrade, T Saha, D Sundaravin, J Garzón, W Zhang, O Popp, SI Hiraga, A Rahjouei, DB Rosen, P Mertins, BT Chait, AD Donaldson, M Di Virgili Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region Elife, 2022-04-13;11(0):. 2022-04-13 [PMID: 35416772] (Western Blot, Human)
• Yang Y, Lu H, Chen C et al. HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia Nature communications 2022-01-14 [PMID: 35031618] (WB)
• Leimbacher PA, Jones SE, Shorrocks AK et al. MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis Mol. Cell 2019-03-18 [PMID: 30898438] (WB, ICC/IF, Human)
• Piao CS, Loane DJ, Stoica B et al. Combined inhibition of cell death induced by apoptosis inducing factor and caspases provides additive neuroprotection in experimental traumatic brain injury. Neurobiol Dis. 2012-06-01 [PMID: 22426396] (IF/IHC, IP, Mouse)
• Wu CY, Kang HY, Yang WL et al. Critical role of monoubiquitination of histone H2AX protein in histone H2AX phosphorylation and DNA damage response. J Biol Chem 2011-09-01 [PMID: 21690091]
• Artus C, Boujrad H, Bouharrour A et al. AIF promotes chromatinolysis and caspase-independent programmed necrosis by interacting with histone H2AX. EMBO J 2010-04-01 [PMID: 20360685] (Mouse)
• Cheok CF, Kua N, Kaldis P, Lane DP. Combination of nutlin-3 and VX-680 selectively targets p53 mutant cells with reversible effects on cells expressing wild-type p53. Cell Death Differ 2010-09-01 [PMID: 20203688] (ICC/IF, Human)
• Cheng WH, Muftic D, Muftuoglu M et al. WRN is required for ATM activation and the S-phase checkpoint in response to interstrand cross-link-induced DNA double-strand breaks. Mol Biol Cell 2008-09-01 [PMID: 18596239]
• Thompson, S L D A Compton. Examining the link between chromosomal instability aneuploidy in human cells. J Cell Biol 180: 665-672. 2008-01-01 [PMID: 18283116]
• Masaki H, Nishida T, Kitajima S et al. Developmental pluripotency-associated 4 (DPPA4) localized in active chromatin inhibits mouse embryonic stem cell differentiation into a primitive ectoderm lineage. J Biol Chem 2007-11-01 [PMID: 17855347]
• Muramatsu Y, Ohishi T, Sakamoto M et al. Cross-species difference in telomeric function of tankyrase 1. Cancer Sci 2007-06-01 [PMID: 17433040]
• Hovest, MG et al. Senescence of human fibroblasts after psoralen photoactivation is mediated by ATR kinase persistent DNA damage foci at telomerase. Mol Biol Cell 17: 1758-1767. 2006-01-01 [PMID: 16436511]
• Masutomi, K et al. The telomerase reverse transcriptase regulates chromatin state DNA damage responses. PNAS 102(23): 8222-8227. 2005-01-01 [PMID: 15928077] (WB, Human)

FAQs for Histone H2AX Antibody (NB100-383). (Showing 1 - 1 of 1 FAQs).

1. Could you provide the detailed immunogen peptide sequence?
   • The epitope which is recognized by NB100-383 maps to the C-terminus of human histone H2AX (Swiss-Prot entry P16104). Unfortunately we are not able to provide any further information than this as the exact immunogen information is considered to be proprietary.

Bioinformatics

• Gene Symbol: H2AX
• Entrez:
  • 3014(Human)
  • 15270(Mouse)
• Uniprot:
  • P16104()