Histidase Antibody - BSA Free

Immunohistochemistry-Paraffin: Histidase Antibody [NBP1-89694] - Staining of human cerebral cortex, liver, skin and testis using Anti-HAL antibody NBP1-89694 (A) shows similar protein distribution across tissues to independent antibody NBP1-89695 (B).

Immunohistochemistry-Paraffin: Histidase Antibody [NBP1-89694] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: Histidase Antibody [NBP1-89694] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.

Staining of human testis shows moderate cytoplasmic positivity.

Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.

• Reactivity: Hu, Mu
• Applications: Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Histidase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Histidase Antibody - BSA Free (NBP1-89694) is a polyclonal antibody validated for use in IHC and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: FVEVVIEGDAMSPDFIPSQPEGVYLYSKYREPEKYIELDGDRLTTEDLVNLGKGRYKIKLTPTAEKRVQKSREVIDSIIKEKTVVYGITTGFGKFARTV
• Predicted Species: Mouse (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HAL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:250
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Histidase Protein (NBP1-89694PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Histidase Antibody - BSA Free

• EC 4.3.1
• EC 4.3.1.3
• HIS
• histidase
• histidine ammonia-lyase
• HSTD

Background

Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAL