Histamine N-Methyltransferase/HNMT Antibody (034) - Azide and BSA Free

Western Blot: Histamine N-Methyltransferase/HNMT Antibody (034) [NBP2-90171] - Anti-HNMT rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Predicted band size:33 kDa. Observed band size:33 kDa.

Immunoprecipitation: Histamine N-Methyltransferase/HNMT Antibody (034) [NBP2-90171] - HNMT was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate. Lane B:0.5 mg Hela Whole Cell Lysate. 2 uL anti-HNMT rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-HNMT rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 33 kDa.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 034
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Histamine N-Methyltransferase/HNMT Antibody (034) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human Histamine N-Methyltransferase/HNMT (Accession#: AAH20677.1; Met1-Ala292).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HNMT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 0.5-2 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Histamine N-Methyltransferase/HNMT Antibody (034) - Azide and BSA Free

• EC 2.1.1.8
• Histamine NMethyltransferase
• Histamine N-Methyltransferase
• HMT
• HNMT
• HNMT-S1
• HNMT-S2

Background

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNMT