HIST3H3 Antibody (2128A) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB, IHC, Flow, ICC/IF
• Clone: 2128A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

HIST3H3 Antibody (2128A) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human HIST3H3 synthetic peptide
• Specificity: Detects human HIST3H3 in Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Intracellular Staining by Flow Cytometry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for HIST3H3 Antibody (2128A) [CoraFluor™ 1]

• H3 histone family, member T
• H3.4
• H3/G
• H3/gH3.4
• H3/T
• H3FT
• H3FTMGC126888
• H3t
• H3tMGC126886
• HIST3H3
• Histone 3, H3
• histone cluster 3, H3
• histone H3.1t

Background

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The HIST3H3 gene is intronless and encodes a 136 amino acid long, 15 kDA histone H3.1t protein that lacks polyA tails and obtain a palindromic termination element. The HIST3H3 gene is located separately from the other H3 genes on the cluster on chromosome 6p22-p21.3. HIST3H3 participates in PKA signaling, development notch signaling pathway, histone modification, and interferon pathway through interactions with genes HIST1H4B, HIST4H4, HIST1H4A, HIST1H4C, and HIST1H4D. HIST3H3 has been researched regarding its role in immunodeficiency, leukemia, prostatitis, huntington's disease, lupus, multiple sclerosis, laryngeal cancer, and lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.