HIP1 Antibody (4B10) - BSA Free

Western Blot: HIP1 Antibody (4B10) [NB300-203] - Western Blot on HeLa whole cell extracts.

• Reactivity: Hu, Mu, Rt(-)
• Applications: WB, ChIP, ICC/IF, IHC, IP, ChIP
• Clone: 4B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

HIP1 Antibody (4B10) - BSA Free Summary

• Immunogen: Human 3'-HIP1 (~65 kDa)
• Localization: Cytoplasm
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HIP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) reported in scientific literature
  • Chromatin Immunoprecipitation reported in scientific literature
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry reported in scientific literature
  • Immunohistochemistry-Paraffin reported in scientific literature
  • Immunoprecipitation reported in scientific literature
  • Western Blot 1:1000
• Application Notes: In Western blot, a band at approximately 115 kDa is seen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 115 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Does not react with rat.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for HIP1 Antibody (4B10) - BSA Free

• HIP-1
• HIP-I
• huntingtin interacting protein 1
• huntingtin-interacting protein 1
• Huntingtin-interacting protein I
• ILWEQ
• MGC126506

Background

One of the four subunits of the histone regulatory complex A, HIP1 is an anti-apoptosis protein in the brain. HIP1 (Huntingtin-interacting protein 1) protects normal neurological tissue from erroneous inflammatory and apoptotic events by linking the actin cytoskeleton and clathrin-mediated endocytosis machinery. It is important in the generation of clathrin coated vesicles in the neuron. However, if deletion of HIP1 occurs, neurological disease is initiated and is correlated to epilepsy, learning disabilities, spinal and testicular defects, hematopoietic issues, and other neurobehavioral abnormalities such as Huntington's disease. HIP1 also interacts with the Hedgehog signaling pathway, and is important in regulating the abundance and scope of Hedgehog signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIP1 Antibody (NB300-203)(11)

Publications using NB300-203

• Krzystek TJ, White JA, Rathnayake R, Thurston L et Al. HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury Autophagy 2022-09-01 [PMID: 36048753]
• Bettencourt C, Miki Y, Piras IS et al. MOBP and HIP1 in multiple system atrophy: New ?-synuclein partners in glial cytoplasmic inclusions implicated in the disease pathogenesis Neuropathology and Applied Neurobiology 2021-08-01 [PMID: 33368549] (Immunoprecipitation, Western Blot, Immunocytochemistry/ Immunofluorescence, Human, Mouse)
• Fontaine SN, Bauer SP, Lin X et al. Replacement of charged and polar residues in the coiled-coiled interface of huntingtin-interacting protein 1 (HIP1) causes aggregation and cell death FEBS Lett 2012-07-23 [PMID: 22835334] (ICC/IF, WB, Human)
• Barker BL, Benovic J. G protein-coupled receptor kinase 5 phosphorylation of hip regulates internalization of the chemokine receptor CXCR4 Biochemistry 2011-08-16 [PMID: 21728385] (WB, Human)
• Bradley SV, Smith MR, Hyun TS, Lucas PC, Li L, Antonuk D, Joshi I, Jin F, Ross TS. Aberrant Huntingtin interacting protein 1 in lymphoid malignancies. Cancer Res;67(18):8923-31. 2007-09-15 [PMID: 17875735]
• Bradley SV, HollEC, Liu GY, Thomas D, Hyun TS, Ross TS. Huntingtin interacting protein 1 is a novel brain tumor marker that associates with epidermal growth factor receptor. Cancer Res;67(8):3609-15. 2007-04-15 [PMID: 17440072] (IHC-P, Human)
• Mills IG et al. Huntingtin interacting protein 1 modulates the transcriptional activity of nuclear hormone receptors. J Cell Biol; 70: 191-200. 2005-01-01 [PMID: 16027218] (Chemotaxis, Human)
• Bradley SV, Oravecz-Wilson KI, Bougeard G, Mizukami I, Li L, Munaco AJ, Sreekumar A, Corradetti MN, Chinnaiyan AM, Sanda MG, Ross TS. Serum antibodies to huntingtin interacting protein-1: a new blood test for prostate cancer. Cancer Res;65(10):4126-33. 2005-05-15 [PMID: 15899803] (WB, Human, Mouse)
• Hyun TS, Rao DS, Saint-Dic D, Michael LE, Kumar PD, Bradley SV, Mizukami IF, Oravecz-Wilson KI, Ross TS. HIP1 and HIP1r stabilize receptor tyrosine kinases and bind 3-phosphoinositides via epsin N-terminal homology domains. J Biol Chem;279(14):14294-306. 2004-04-02 [PMID: 14732715] (ICC/IF, Human)
• Rao, DS et al. Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival. J Clin Invest;110(3):351-60. 2002-08-01 [PMID: 12163454]
• Rao DS, Chang JC, Kumar PD, Mizukami I, Smithson GM, Bradley SV, Parlow AF, Ross TS. Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors. Mol Cell Biol;21(22):7796-806. 2001-11-01 [PMID: 11604514] (IP, ICC/IF, WB, Mouse, Human)

Bioinformatics

• Gene Symbol: HIP1
• Entrez:
  • 3092(Human)
• Uniprot:
  • O00291(Human)