Hip Antibody (HL1737) - Azide and BSA Free

Immunohistochemistry-Paraffin: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] - HHIP antibody [HL1737] detects HHIP protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HHIP stained by HHIP antibody [HL1737] (NBP3-25505) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1737] (NBP3-25505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemistry-Paraffin: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] - HHIP antibody [HL1737] detects HHIP protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HHIP stained by HHIP antibody [HL1737] (NBP3-25505) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, IHC
• Clone: HL1737
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Hip Antibody (HL1737) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Hip Antibody (HL1737) - Azide and BSA Free (NBP3-25505) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Hip. The exact sequence is proprietary.
• Source: HEK293
• Predicted Species: Rat (98%), Bovine (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HHIP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (82%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Hip Antibody (HL1737) - Azide and BSA Free

• FLJ90230
• hedgehog interacting protein
• hedgehog-interacting protein
• HHIP
• Hip
• HIPFLJ20992

Background

Hhip encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signalling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HHIP