HINT2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: HINT2 Antibody [NBP2-56156] - Staining of human cell line U-2 OS shows localization to mitochondria.

Immunohistochemistry-Paraffin: HINT2 Antibody [NBP2-56156] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.

Immunohistochemistry-Paraffin: HINT2 Antibody [NBP2-56156] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: HINT2 Antibody [NBP2-56156] - Staining of human fallopian tube shows moderate granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: HINT2 Antibody [NBP2-56156] - Staining of human fallopian tube, gastrointestinal, kidney and testis using Anti-HINT2 antibody NBP2-56156 (A) shows similar protein distribution across tissues to independent antibody NBP1-86024 (B).

Immunohistochemistry-Paraffin: HINT2 Antibody [NBP2-56156] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HINT2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GVTDGNEVAKAQQATPGGAAPTIFSRILDKSLPADILYEDQQCLVFRDVAPQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HINT2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  HINT2 Recombinant Protein Antigen (NBP2-56156PEP)

Reactivity Notes

Mouse 88%, Rat 88%

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HINT2 Antibody - BSA Free

• EC 3.-
• HINT-2
• HINT-3
• histidine triad nucleotide binding protein 2
• histidine triad nucleotide-binding protein 2, mitochondrial
• HIT-17
• HIT-17kDa
• PKCI-1-related HIT protein
• protein kinase C inhibitor-2

Background

Histidine triad proteins, such as HINT2, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 [PubMed 12119013]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HINT2