HINT1 Antibody

Images

 
Western Blot: HINT1 Antibody [NBP1-76944] - Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: HINT1 Antibody [NBP1-76944] - Jurkat cells with HINT1 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HINT1 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the center of the human HINT1. The immunogen is located within the last 50 amino acids of HINT1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HINT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Control Peptide
HINT1 Peptide (NBP1-76944PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HINT1 Antibody

  • Adenosine 5'-monophosphoramidase
  • EC 3.-
  • HINT
  • histidine triad nucleotide binding protein 1
  • histidine triad nucleotide-binding protein 1
  • histidine triad nucleotide-binding protein
  • PKCI1
  • PKCI-1FLJ30414
  • PRKCNH1FLJ32340
  • Protein kinase C inhibitor 1
  • Protein kinase C-interacting protein 1

Background

Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and –hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5'-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HINT1
Uniprot