hHR23b Antibody

Western Blot: hHR23b Antibody [NBP2-16825] - Wild-type (WT) and hHR23b knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hHR23b antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: hHR23b Antibody [NBP2-16825] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat C: Raji 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: hHR23b Antibody [NBP2-16825] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: hHR23b Antibody [NBP2-16825] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

hHR23b Antibody Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit hHR23b Antibody (NBP2-16825) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human hHR23b. The exact sequence is proprietary.
• Localization: Nucleus; Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAD23B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for hHR23b Antibody

• HHR23B
• HR23BUV excision repair protein RAD23 homolog B
• P58
• RAD23 (S. cerevisiae) homolog B
• RAD23 homolog B (S. cerevisiae)
• RAD23, yeast homolog of, B
• XP-C repair complementing complex 58 kDa
• XP-C repair complementing protein
• XP-C repair-complementing complex 58 kDa protein

Background

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RAD23B
• Uniprot:
  • P54727(Human)