HGFR/c-MET Antibody [Unconjugated]

Images

 
HGF R/c‑MET was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive control, left panel) and U937 human histiocytic lymphoma cell line (negative control, right panel) using Goat ...read more
HGF R/c‑MET was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse HGF R/c‑MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) at 15 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

HGFR/c-MET Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse HGF R/c-MET
Glu25-Asn929
Accession # P16056
Specificity
Detects mouse HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) HGF R and less than 1% cross-reactivity with rhMSP R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MET
Endotoxin Note
<0.01 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 4 Reviews rated 4.3
using
AF527 in the following applications:

Publications
Read Publications using
AF527 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HGFR/c-MET Antibody [Unconjugated]

  • AUTS9
  • cMET
  • c-MET
  • EC 2.7.10
  • EC 2.7.10.1
  • hepatocyte growth factor receptor
  • HGF R
  • HGF receptor
  • HGF/SF receptor
  • HGFR
  • Met (c-Met)
  • met proto-oncogene (hepatocyte growth factor receptor)
  • met proto-oncogene tyrosine kinase
  • MET
  • oncogene MET
  • Proto-oncogene c-Met
  • RCCP2
  • Scatter factor receptor
  • SF receptor
  • Tyrosine-protein kinase Met

Background

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular  alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). An alternately spliced form of mouse HGF R lacks a cytoplasmic juxtamembrane region important for regulation of signal transduction (5, 6). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 7). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (8, 9). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (10). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin  alpha 6/ beta 4, plexins B1, 2, 3, and MSP R/Ron (11-18). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (11-18). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (11, 15, 16). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (19). Genetic polymorphisms, chromosomal translocation,
over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, mouse HGF R shares 87%, 87%, and 94% amino acid sequence identity with canine, human, and rat HGF R, respectively.

  1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
  2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
  4. Chan, A.M. et al. (1988) Oncogene 2:593.
  5. Lee, C.-C. and K.M. Yamada (1994) J. Biol. Chem. 269:19457.
  6. Lee, C.-C., et al. (1995) J. Biol. Chem. 270:507.
  7. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
  8. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
  9. Ponzetto, C. et al. (1994) Cell 77:261.
  10. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
  11. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
  12. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
  13. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
  14. Wang, X. et al. (2002) Mol. Cell 9:411.
  15. Trusolino, L. et al. (2001) Cell 107:643.
  16. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
  17. Conrotto, P. et al. (2004) Oncogene 23:5131.
  18. Follenzi, A. et al. (2000) Oncogene 19:3041.
  19. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HGFR/c-MET Antibody (AF527)(21)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 9 applications: ELISA Development, ELISA Development (Capture), ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
ELISA Development (Capture)
(1)
ICC
(4)
IHC
(1)
IHC-Fr
(3)
IHC-P
(4)
Immunoprecipitation
(1)
Neutralization
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(17)
Rat
(3)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using AF527 Applications Species
J Mi, E Hooker, S Balog, H Zeng, DT Johnson, Y He, EJ Yu, H Wu, V Le, DH Lee, J Aldahl, ML Gonzalgo, Z Sun Activation of hepatocyte growth factor/MET signaling initiates oncogenic transformation and enhances tumoraggressiveness in the murine prostate J. Biol. Chem., 2018;0(0):. 2018 [PMID: 30401749] (IHC-P, Mouse) IHC-P Mouse
K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams Pulmonary pericytes regulate lung morphogenesis Nat Commun, 2018;9(1):2448. 2018 [PMID: 29934496] (IHC-P, Mouse) IHC-P Mouse
Hepatocyte Growth Factor-c-MET Signaling Mediates the Development of Nonsensory Structures of the Mammalian Cochlea and Hearing J Neurosci, 2016;36(31):8200-9. 2016 [PMID: 27488639] (IHC-Fr, Mouse) IHC-Fr Mouse
Rachel Barrow-McG Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes Nat Commun, 2016;7(0):11942. 2016 [PMID: 27336951] (Western Blot, Mouse) Western Blot Mouse
Lia Tesfay Receptor Tyrosine Kinase Met Promotes Cell Survival via Kinase-Independent Maintenance of Integrin ?3?1 Mol Biol Cell, 2016;0(0):. 2016 [PMID: 27307589] (Western Blot, Human) Western Blot Human
Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson A, Wauters E, Walmsley S, Prenen H, Granot Z, Casazza A, Mazzone M MET is required for the recruitment of anti-tumoural neutrophils. Nature, 2015;522(7556):349-53. 2015 [PMID: 25985180] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux. PLoS ONE, 2015;10(5):e0125797. 2015 [PMID: 25938594] (IHC, Mouse) IHC Mouse
Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling. Nat Commun, 2015;6(0):6776. 2015 [PMID: 25873032] (IHC-P, Mouse) IHC-P Mouse
Wei W, Howard P, Macarak E Recombinant insulin-like growth factor-1 activates satellite cells in the mouse urethral rhabdosphincter. BMC Urol, 2013;13(0):62. 2013 [PMID: 24279352] (IHC-Fr, Mouse) IHC-Fr Mouse
Gordin M, Tesio M, Cohen S c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74. J. Immunol., 2010;185(4):2020-31. 2010 [PMID: 20639480] (Western Blot, Mouse) Western Blot Mouse
Show All 21 Publications.

Reviews for HGFR/c-MET Antibody (AF527) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Mouse.

Reviews using AF527:
Filter by Applications
IHC-Fr
(2)
WB
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23074208
SpeciesMouse
 
reviewed by:
Anonymous
WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23509284
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23509284

Details

Detection NotesSee PMID 23509284

Comments

CommentsPublished: See PMID 23509284Independent review from antibodyresource.com
 
reviewed by:
Anonymous
IHC-P Mouse 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24218051
SpeciesMouse
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 24279352
SpeciesMouse

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Secondary Antibodies

 

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Biotin BAF527

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

 
Anonymous
01/08/2015
Application: IHC-Fr
Species: Mouse

 
Anonymous
01/08/2015
Application: WB
Species: Mouse

 
Anonymous
01/08/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol MET
Uniprot