HEY1 Antibody (3B3) - Azide and BSA Free

Western Blot: HEY1 Antibody (3B3) [H00023462-M01] - analysis of HEY1 expression in transfected 293T cell line by HEY1 monoclonal antibody (M01), clone 3B3. Lane 1: HEY1 transfected lysate (32.613 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: HEY1 Antibody (3B3) [H00023462-M01] - Analysis of monoclonal antibody to HEY1 on HeLa cell . Antibody concentration 10 ug/ml.

ELISA: HEY1 Antibody (3B3) [H00023462-M01] - Detection limit for recombinant GST tagged HEY1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HEY1 Antibody (3B3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HEY1 Antibody (3B3) - Azide and BSA Free (H00023462-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-HEY1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: HEY1 (NP_036390, 121 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DYRSLGFRECLAEVARYLSIIEGLDASDPLRVRLVSHLNNYASQREAASGAHAGLGHIPWGTVFGHHPHIAHPLLLPQNGHGNAGTTASPTEPHHQGRLG
• Specificity: hairy/enhancer-of-split related with YRPW motif 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HEY1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HEY1 Antibody (3B3) - Azide and BSA Free

• basic helix-loop-helix protein OAF1
• BHLHb31
• Cardiovascular helix-loop-helix factor 2
• CHF-2
• CHF2hHRT1
• Class B basic helix-loop-helix protein 31
• Hairy and enhancer of split-related protein 1
• hairy/enhancer-of-split related with YRPW motif 1
• hairy/enhancer-of-split related with YRPW motif protein 1
• Hairy-related transcription factor 1
• HERP2HES-related repressor protein 1
• HESR-1
• HESR1MGC1274
• HES-related repressor protein 2
• HRT1
• HRT-1OAF1

Background

This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HEY1 Antibody (H00023462-M01)(2)

Publications using H00023462-M01

• Hrstka SC, Li X, Nelson TJ. et al. NOTCH1-dependent nitric oxide signaling deficiency in hypoplastic left heart syndrome revealed through patient-specific phenotypes detected in bioengineered cardiogenesis. Stem Cells. 2017-01-01 [PMID: 28142228] (WB, Human)
• Tsuru A, Setoguchi T, Matsunoshita Y et al. Hairy/enhancer-of-split related with YRPW motif protein 1 promotes osteosarcoma metastasis via matrix metallopeptidase 9 expression. Br J Cancer. 2015-03-05 [PMID: 25742474]

Bioinformatics

• Gene Symbol: HEY1
• Entrez:
  • 23462(Human)
• OMIM:
  • 602953(Human)
• Uniprot:
  • NP_036390(Human)