Rat Hexokinase 1 ELISA Kit (Colorimetric)

ELISA: Rat Hexokinase 1 ELISA Kit (Colorimetric) [NBP2-82213] - Samples were spiked with high concentrations of Rat Hexokinase 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Hexokinase 1 ELISA Kit (Colorimetric) [NBP2-82213] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 56.25 pg/mL

Rat Hexokinase 1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat Hexokinase 1 in samples. No Significant cross-reactivity or interference between Rat Hexokinase 1 and analogues was observed.
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 56.25 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 11%
• Intra-Assay: CV% < 11%
• Spike Recovery: average = 95% for serum; average = 97% for EDTA plasma; average = 98% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HK1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Hexokinase 1 ELISA Kit (Colorimetric)

• Brain form hexokinase
• EC 2.7.1
• EC 2.7.1.1
• glycolytic enzyme
• Hexokinase 1
• Hexokinase type I
• hexokinase-1
• HK I
• HK1
• HK1-ta
• HK1-tb
• HK1-tc
• HKI
• HXK1

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Hexokinase 1 ELISA Kit (NBP2-82213). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HK1