Recombinant Human Hexokinase 1 His Protein

SDS-Page: Recombinant Human Hexokinase 1 Protein [NBC1-18390] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Product Discontinued

Recombinant Human Hexokinase 1 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-917 of Human Hexokinase 1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISETLKTRG IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA VVDKIRENRG LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG AALITAVGVR LRTEASS

• Details of Functionality: Specific activity is > 8,000 pmol/min/ug. One unit will convert 1 pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH 7.5 at 37C.
• Source: E. coli
• Protein/Peptide Type: Biologically Active Protein
• Gene: HK1
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 104.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human Hexokinase 1 His Protein

• Brain form hexokinase
• EC 2.7.1
• EC 2.7.1.1
• glycolytic enzyme
• Hexokinase 1
• Hexokinase type I
• hexokinase-1
• HK I
• HK1
• HK1-ta
• HK1-tb
• HK1-tc
• HKI
• HXK1

Background

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency. Recombinant human Hexokinase1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Publications for Hexokinase 1 Biologically Active Protein (NBC1-18390)(2)

Publications using NBC1-18390

• Peng S, Gerasimenko JV, Tsugorka TM et al. Galactose protects against cell damage in mouse models of acute pancreatitis J Clin Invest. [PMID: 29893744] (BA, Mouse)
• Norman GL, Yang CY, Ostendorff HP et al. Anti-kelch-like 12 and anti-hexokinase 1: novel autoantibodies in primary biliary cirrhosis. Liver Int. 2014-09-19 [PMID: 25243383] (ELISA)
  
  Details:
  Hexokinase 1 protein used in the ELISA assay for the detection of anti-hexokinase 1: novel autoantibodies in the serum samples from primary biliary cirrhosis

Bioinformatics

• Gene Symbol: HK1