Hexokinase 1 Antibody (21)

Product Discontinued

Hexokinase 1 Antibody (21) Summary

• Immunogen: Rat Type I hexokinase.
• Specificity: Recognizes a segmental epitope located between residues 191-209 of the Type I hexokinase sequence.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HK1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:500-1:2000
• Application Notes: Immunolocalization of Type I hexokinase at dilution of 1:500-1:2,000.

Reactivity Notes

Bovine, Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for Hexokinase 1 Antibody (21)

• Brain form hexokinase
• EC 2.7.1
• EC 2.7.1.1
• glycolytic enzyme
• Hexokinase 1
• Hexokinase type I
• hexokinase-1
• HK I
• HK1
• HK1-ta
• HK1-tb
• HK1-tc
• HKI
• HXK1

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HK1
• Uniprot:
  • P19367()
  • P05708(Rat)