HES5 Antibody - BSA Free

Western Blot: HES5 Antibody - BSA Free [NBP3-46125] - Rat brain tissue were subjected to SDS PAGE followed by western blot with (HES5 Antibody) at dilution of 1:300

Immunohistochemistry: HES5 Antibody - BSA Free [NBP3-46125] - Immunohistochemistry of paraffin-embedded mouse brain tissue slide using (HES5 Antibody) at dilution of 1:50

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HES5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HES5 Antibody - BSA Free (NBP3-46125) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: hairy and enhancer of split 5(Drosophila). (Uniprot# Q5TA89)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HES5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HES5 Antibody - BSA Free

• BHLHB38
• bHLHb38Class B basic helix-loop-helix protein 38
• hairy and enhancer of split 5 (Drosophila)
• Hairy and enhancer of split 5
• transcription factor HES-5

Background

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HES5